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Evaluation of phenolic substances content in plant material
Hollá, Marcela ; Sklenářová, Hana (advisor) ; Hroboňová, Katarína (referee) ; Štěrbová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Marcela Hollá Supervisor: assoc. prof. PharmDr. Hana Sklenářová, Ph.D. Consultant: prof. RNDr. Dalibor Šatínský, Ph.D. Title of Doctoral Thesis: Evaluation of phenolic substances content in plant material Scientific community has always been interested in the research of natural substances. The main reasons are a huge diversity of substances themselves and their significant impact on human health. The development of new analytical and extraction methods, including new approaches for sample preparation, enable obtaining more detailed knowledge about the structure and biological properties of plant substances. A significant group consists of substances with antioxidant effects, such as phenolic compounds, which are an important part of human food. The presented dissertation thesis deals with determination of selected groups of natural substances from the point of view of specific requirements for chromatographic separation, choice of detection technique with regard to the selectivity and sensitivity of the determination, as well as the possibility of applying modern and green extraction approaches. The first part of the presented work is devoted to the issue of determination of selected plant...
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Development and optimization of chromatographic methods suitable for clinical application
Jenčo, Jaroslav ; Solich, Petr (advisor) ; Hroboňová, Katarína (referee) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Jaroslav Jenčo Supervisor: prof. RNDr. Petr Solich, CSc. Consultant: doc. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of dissertation thesis: Development and optimization of chromatographic methods suitable for clinical application The presented dissertation thesis is divided into several parts, copying the workplaces, which I gradually worked on. The unifying element is the development and optimization of methods for the preparation of samples of biologically active substances and their subsequent analysis using separation methods. The theoretical part characterizes the target substances, from a biochemical, physicochemical, and analytical point of view. It describes the basic characteristics of extraction procedures and presents an overview of the methods used to determine the substances described in the thesis. In the first, main experimental part, of the dissertation thesis the development, optimization, and validation of an analytical method for chromatographic analysis of vitamin B1, its biologically active forms and the active form of vitamin B6 in blood is described. It illustrates the selection of sample preparation techniques, the optimization of the target methodology...
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Development and optimization of chromatographic methods suitable for clinical application
Jenčo, Jaroslav ; Solich, Petr (advisor) ; Hroboňová, Katarína (referee) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department: Department of Analytical Chemistry Candidate: Mgr. Jaroslav Jenčo Supervisor: prof. RNDr. Petr Solich, CSc. Consultant: doc. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of dissertation thesis: Development and optimization of chromatographic methods suitable for clinical application The presented dissertation thesis is divided into several parts, copying the workplaces, which I gradually worked on. The unifying element is the development and optimization of methods for the preparation of samples of biologically active substances and their subsequent analysis using separation methods. The theoretical part characterizes the target substances, from a biochemical, physicochemical, and analytical point of view. It describes the basic characteristics of extraction procedures and presents an overview of the methods used to determine the substances described in the thesis. In the first, main experimental part, of the dissertation thesis the development, optimization, and validation of an analytical method for chromatographic analysis of vitamin B1, its biologically active forms and the active form of vitamin B6 in blood is described. It illustrates the selection of sample preparation techniques, the optimization of the target methodology...
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