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Enzymatic Sensor of Biogenic Amines with Optical Oxygen Transducer
 Maixnerová, Lucie ; Horvitz, Alexandar ; Kuncová, Gabriela ; Přibyl, M. ; Šebela, M. ; Koštejn, Martin
The biogenic amines (BA) are formed by the decarboxylation of amino acids. Because of their impact on human health, they have been used as markers for assessing the freshness and quality of a wide variety of protein-containing products. We studied biosensor for BA with an optical oxygen transducer that contained a diamine oxidase from pea and ruthenium complex. Both active components were incorporated in chemically stable polymer to form sensitive films thickness of 100 - 200 μm. With aim to optimize a composition and manufacturing of the biosensor we developer a mathematical model of processes inside the sensing film. The developed mathematical model revealed qualitative dependencies of the biosensor behavior (sensitivity, response time and dynamic range) on the (i) thickness of the sensitive layer, (ii) concentrations of substrates in the solution, (iii) enzyme activity in the layer and (iv) layer permeability for the substrates.
Fulltext: content.csg - Download fulltextPDF
Plný tet: SKMBT_C22015011213553 - Download fulltextPDF
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