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Carbon nanoparticles as promising components of materials for bone tissue engineering
Grausová, Ľubica
Recently, nanotechnology in tissue engineering has become a very important field of study. Many different materials of nanoscale roughness have been studied for their potential use in the regeneration of various tissues. The reason is that nanostructured materials imitate the architecture of natural extracellular matrix and thus support cell adhesion, growth and differentiation. Our investigations were focused on the influence of fullerene layers, carbon nanotube-terpolymer composites and nanocrystalline diamond layers on the adhesion, growth and differentiation of human osteoblast-like MG 63 cells. Each of these materials supported colonization with cells. On continuous fullerene C60 layers, deposited on composites with the carbon matrix reinforced with carbon fibres, the cell population density was lower than on non-coated composites, but MG 63 cells were well-spread with well-developed vinculin-containing focal adhesion plaques and a beta-actin cytoskeleton. On composites of carbon nanotubes with a terpolymer of polytetrafluoroethylene, polypropylene and polyvinyldifluoride, the adhesion, spreading, formation of focal adhesion plaques and actin cytoskeleton, viability and cell growth were markedly improved in comparison with pure terpolymer. At the same time, these cells did not show significant immune...
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Carbon nanoparticles as promising components of materials for bone tissue engineering
Grausová, Ľubica
Recently, nanotechnology in tissue engineering has become a very important field of study. Many different materials of nanoscale roughness have been studied for their potential use in the regeneration of various tissues. The reason is that nanostructured materials imitate the architecture of natural extracellular matrix and thus support cell adhesion, growth and differentiation. Our investigations were focused on the influence of fullerene layers, carbon nanotube-terpolymer composites and nanocrystalline diamond layers on the adhesion, growth and differentiation of human osteoblast-like MG 63 cells. Each of these materials supported colonization with cells. On continuous fullerene C60 layers, deposited on composites with the carbon matrix reinforced with carbon fibres, the cell population density was lower than on non-coated composites, but MG 63 cells were well-spread with well-developed vinculin-containing focal adhesion plaques and a beta-actin cytoskeleton. On composites of carbon nanotubes with a terpolymer of polytetrafluoroethylene, polypropylene and polyvinyldifluoride, the adhesion, spreading, formation of focal adhesion plaques and actin cytoskeleton, viability and cell growth were markedly improved in comparison with pure terpolymer. At the same time, these cells did not show significant immune...
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Carbon nanoparticles as promising components of materials for bone tissue engineering
Grausová, Ľubica ; Bačáková, Lucie (advisor) ; Mikšík, Ivan (referee) ; Balík, Karel (referee)
Recently, nanotechnology in tissue engineering has become a very irnportant field of study. Many different materials of nanoscale roughness have been studied for their potential use in the regeneration of various tissues. The reason is that nallostructured materials imitate the architecture of natural extracellular matrix and thus support cell adhesion, growth and differentiation. Our investigations were focused on the influence of fullerene layers, carbon nanotube-terpolymer composites and nanocrystalline diamond layers on the adhesion. growth and differentiation of human osteoblast-like MG 63 cells. Each of these materials supported colonization with cells. On continuous fullerene C6s layers, deposited on composites with the carbon matrir reinforced with carbon fibres, the cell population density was lower than on non-coated composites. but MG 63 cells were well-spread w'ith rvell-developed vinculin-containing focal adhesiort plaques and a beta- actin cy'toskeleton. On contposites of carbon naliotubes u ith a terpolymer of poll.tetrafluoroethvlene. poly propy lene and polyvinyldifluoride, the adhesion, spreading. formation oť tbcal adhesion plaques and actin cytoskeleton, viability and cell grouth u ere markedly improved in comparison with pure terpolymer. At the same time, these cells did not...
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