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Celiac disease-related inflammatory factors and their effect on peripheral blood monocytes
Golovina, Elena ; Daňková, Pavlína (advisor) ; Čepek, Pavel (referee)
Introduction: Celiac disease is an organ-specific autoimmune disease that results in loss of oral tolerance of gluten in genetically predisposed individuals. Increased levels of inflammatory cytokines (eg IFN-γ, TNF-α, IL-6) in the small intestine and peripheral blood have been observed in patients with celiac disease. These cytokines have the ability to increase the expression of interferon regulatory factor 1 (IRF1), which is a potential player in the pathogenesis of celiac disease. IRF1 plays a role in apoptosis, differentiation and anti- inflammatory response of various immune cells. Monocytes are an important component of the immune system. A wide variety of their functions allow them to exertmembrane receptors, whose expression may cause, among other things, by IRF1. To clarify the effects of IRF1 on the pathogenesis of celiac disease we have focused on circulating monocytes in this pilot study and (i) detection changes in the monocytic expression of IRF1 mRNA in inflammatory environments in patients with celiac disease (recent, rCD and adherent one year and longer gluten-free diet, CD-GFD), and ii) subsequently to reveal the effect of IRF1 on monocytes by apoptosis markers (CD95) and anti-inflammatory responses markers (CD163 and IL-10). Material and methods: The study includes 15 patients...
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