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The Separation of Gastric Aspartic Proteases Using Affinity Chromatography
Frýdlová, Jana
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are produced by gastric mucosa as inactive pepsinogens (pepsinogen A and pepsinogen C) that differ in their physico-chemical and immunological properties. Both pepsinogens consist of molecular variants, isozymogens. Pepsinogens are activated to the corresponding pepsins in the acidic environment of the gastric lumen. The levels of pepsinogens in serum reflect the morphological and functional status of gastric mucosa. The levels of pepsinogens are associated with gastric diseases (e.g. gastric cancer, gastritis). For a diagnostic purpose, the ratio of pepsinogen A to pepsinogen C not only in the gastric mucosa but also in serum is relevant. The gastric cancer patients or the atrophic gastritis patients have significantly lower ratio of serum pepsinogen A to pepsinogen C in comparison with the healthy subjects. In addition, proportion of individual isozymogens reflects the type of the gastric disease. Determination of changes in pepsinogen levels could be useful for early diagnostics of gastric diseases mentioned above. A subject of this Ph.D. thesis is a part of a long-term investigation that focuses on the elaboration of methods for the separation of gastric aspartic proteases that would be suitable for...
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The Separation of Gastric Aspartic Proteases Using Affinity Chromatography
Frýdlová, Jana
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are produced by gastric mucosa as inactive pepsinogens (pepsinogen A and pepsinogen C) that differ in their physico-chemical and immunological properties. Both pepsinogens consist of molecular variants, isozymogens. Pepsinogens are activated to the corresponding pepsins in the acidic environment of the gastric lumen. The levels of pepsinogens in serum reflect the morphological and functional status of gastric mucosa. The levels of pepsinogens are associated with gastric diseases (e.g. gastric cancer, gastritis). For a diagnostic purpose, the ratio of pepsinogen A to pepsinogen C not only in the gastric mucosa but also in serum is relevant. The gastric cancer patients or the atrophic gastritis patients have significantly lower ratio of serum pepsinogen A to pepsinogen C in comparison with the healthy subjects. In addition, proportion of individual isozymogens reflects the type of the gastric disease. Determination of changes in pepsinogen levels could be useful for early diagnostics of gastric diseases mentioned above. A subject of this Ph.D. thesis is a part of a long-term investigation that focuses on the elaboration of methods for the separation of gastric aspartic proteases that would be suitable for...
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The Separation of Gastric Aspartic Proteases Using Affinity Chromatography
Frýdlová, Jana ; Kučerová, Zdenka (advisor) ; Barthová, Jana (referee) ; Jonáková, Věra (referee)
Human gastric juice contains mainly aspartic proteases - pepsin A and pepsin C. Both pepsins are produced by gastric mucosa as inactive pepsinogens (pepsinogen A and pepsinogen C) that differ in their physico-chemical and immunological properties. Both pepsinogens consist of molecular variants, isozymogens. Pepsinogens are activated to the corresponding pepsins in the acidic environment of the gastric lumen. (...) A subject of this Ph.D. thesis is a part of a long-term investigation that focuses on the elaboration of methods for the separation of gastric aspartic proteases that would be suitable for monitoring of their changes in mentioned diseases. This thesis was mainly focused on preparation of affinity sorbents suitable for separation of pepsins and pepsinogens. The choice of ligands was based on the substrate N-acetyl-L-phenylalanyl-3,5-diiodo-L-tyrosine that is used to differentiate pepsin A and pepsin C. The following three affinity sorbents were prepared: iodinated L-tyrosine-Sepharose, 3,5-diiodo-L-tyrosine- Sepharose, and N-acetyl-L-phenylalanine-Sepharose. The basic characteristics of the prepared affinity sorbents were determined using the model enzyme (porcine pepsin A). The comparison of the chromatographic behavior of porcine pepsin A and its complex with pepstatine A showed that the enzyme...
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Marketing Communication in Pharmaceutical Industry: GS CONDRO FORTE
Frýdlová, Jana ; Lešetický, Ondřej (advisor) ; Jankůj, Miroslav (referee)
The aim of this thesis is to present the theoretical attributes of marketing communications, as part of the marketing mix and in particular in relation to specific areas of the pharmaceutical industry. The practical part is focused on pharmaceutical product marketing communication towards the ultimate consumer. For this goal I chose Green pharmaceutical company - Swan Pharmaceuticals CR, as a nutritional supplement for her joints Condro Forte GS.
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