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Diagnostics of fungal disease of nails, skin and hair
Douděrová, Aneta ; Paterová, Pavla (advisor) ; Voxová, Barbora (referee)
Author: Aneta Douděrová Title of the bachelor thesis: Diagnostics of fungal disease of nails, skin and hair Charles University in Prague, Faculty of Pharmacy in Hradec Králové Degree course: Medical laboratory technician The Bachelor thesis on the topic "Diagnostics of fungal disease of nails, skin and hair" is search retrieval. The background is explanation of fungal diseases of the skin and skin adnexe, their classification, causes, clinical manifestations and treatment. There are also the agents of fungal diseases and laboratory diagnosis - microscopy, cultivation, test of antifungal drug resistence and identification micromycetes. It examines sampling tools and sampling techniques for microscopy and cultivation. The chapters are completed by images of fungal diseases and cultivation on agar, which are important for diagnosis. Keywords: Dermatophytes, yeasts, opportunist hyphomycetes, laboratory diagnosis, superficial mycoses, dermatofytosis, tinea, candidiasis, treatment, antimycotics.
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Radiosensitization of non-small cell lung cancer cell line using autophagy inhibitor Lys05
Douděrová, Aneta ; Macháček, Miloslav (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Aneta Douděrová Supervisor: RNDr. Miloslav Macháček, PhD. Consultants: pplk. doc. PharmDr. Aleš Tichý, PhD. Title of diploma thesis: Radiosensitization of non-small cell lung cancer cell line using autophagy inhibitor Lys05 Nowadays, tumor diseases belong to the world's main causes of death. Research in the area of tumor treatment deals with the development of new methods that improve the health of patients. The aim of my diploma thesis was to find out if the usage of autophagy inhibitors in combination with ionizing radiation (IR) contributed to radiosensitization of non-small cell lung cancer cells H1299. It was based on an assumption that autophagy was a cytoprotective mechanism leading to a cell survival during stress conditions such as IR. In order to examine qualitative changes of H1299 cells pre-treated by the new autophagy inhibitor Lys05 or well known autophagy inhibitor Bafilomycin A1 we used fluorescence microscopy in combination with green staining by LysoSensor green DND-189. The intensity of fluorescence was quantified by flow cytometry. The results have shown that pre-treatment by autophagy inhibitors in combination with IR led to morphological changes of cells and to...
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Radiosensitization of non-small cell lung cancer cell line using autophagy inhibitor Lys05
Douděrová, Aneta ; Macháček, Miloslav (advisor) ; Jansová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Aneta Douděrová Supervisor: RNDr. Miloslav Macháček, PhD. Consultants: pplk. doc. PharmDr. Aleš Tichý, PhD. Title of diploma thesis: Radiosensitization of non-small cell lung cancer cell line using autophagy inhibitor Lys05 Nowadays, tumor diseases belong to the world's main causes of death. Research in the area of tumor treatment deals with the development of new methods that improve the health of patients. The aim of my diploma thesis was to find out if the usage of autophagy inhibitors in combination with ionizing radiation (IR) contributed to radiosensitization of non-small cell lung cancer cells H1299. It was based on an assumption that autophagy was a cytoprotective mechanism leading to a cell survival during stress conditions such as IR. In order to examine qualitative changes of H1299 cells pre-treated by the new autophagy inhibitor Lys05 or well known autophagy inhibitor Bafilomycin A1 we used fluorescence microscopy in combination with green staining by LysoSensor green DND-189. The intensity of fluorescence was quantified by flow cytometry. The results have shown that pre-treatment by autophagy inhibitors in combination with IR led to morphological changes of cells and to...
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Diagnostics of fungal disease of nails, skin and hair
Douděrová, Aneta ; Paterová, Pavla (advisor) ; Voxová, Barbora (referee)
Author: Aneta Douděrová Title of the bachelor thesis: Diagnostics of fungal disease of nails, skin and hair Charles University in Prague, Faculty of Pharmacy in Hradec Králové Degree course: Medical laboratory technician The Bachelor thesis on the topic "Diagnostics of fungal disease of nails, skin and hair" is search retrieval. The background is explanation of fungal diseases of the skin and skin adnexe, their classification, causes, clinical manifestations and treatment. There are also the agents of fungal diseases and laboratory diagnosis - microscopy, cultivation, test of antifungal drug resistence and identification micromycetes. It examines sampling tools and sampling techniques for microscopy and cultivation. The chapters are completed by images of fungal diseases and cultivation on agar, which are important for diagnosis. Keywords: Dermatophytes, yeasts, opportunist hyphomycetes, laboratory diagnosis, superficial mycoses, dermatofytosis, tinea, candidiasis, treatment, antimycotics.
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