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Bílkoviny krevní plazmy ovcí
DŘÍZHALOVÁ, Blanka
Plasma protein shave many important functions in the organism. Gamma globulins are carriers of immunoglobulins which play animportant role in the immune response. Their contentis primarily given by the health burden of the organism. The aimofthe study was to determine the individual protein fractions in the blood plasma of ewes and lambs, comparemutual relations between total plasma proteins and thein variol factions and assess the concentrations of individual plasma proteins, mainly globulin, in connection with the aktivity of the thyroidgland, physiological state, and increments. The sampling was carried out in the spring (25.3.) and the autumn (14.10) 2013. The individual protein fractions were determined from the serum by the means of electrophoresis. The results show that the concentration of proteins in the blood plasma of bothewes and ewe lambs and ram lambs was high due to haemoconcentration, heat stress during sampling, grazing young green forage, comprising a large number of protein aceous substances, and increasing demands on energy for milk production, especially in the early stagesoflactation. Theconcentrationofproteins in theblood plasma oflambspertains to thegrowthproduction. It establishes a correlation between the concentration of plasma proteins and the aktivity of the thyroidgland. It also confirmes higher concentrations of plasma proteins of lambs correlating to thein higher daily gain. Due to the high concentration of total protein in the plasma, the level of its fractions was high as well. After the conversion to a percentage, the level of - globulins in blood plasma in all categories was within normal limits in the range of 14-27%. The concentration of - globulins in blood plasma increased in relation to the parasitological findings coccidia of the genus Eimeria and gastrointestinal nematodes. Relations between the kontent of plasma proteins in the blood of the lambs and ewe sobserved were in most casespositive. There was a strong dependence between the total protein and globulins. Even among other fractions of plasma proteins and globulins correlation coefficient was almou always positive.
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The thyroid gland activity of sheep
DŘÍZHALOVÁ, Blanka
In the theoretical part of my thesis, I describe the thyroid, its importance, anatomy, creation of hormones and their regulation. There is also summarized the knowledge of external environment influences on the thyroid such as nutrition, climatic conditions or seasons and internal factors influences, mainly breed, efficiency and age. The practical part is focused on the evaluation of the thyroid activity of free-range ewes and lambs in the spring and autumn of 2013. The thesis also deals with the evaluation of possible relevance between the content of thyroid hormones in the blood serum and the physiological state of the sheep. Thanks to the correlation between TSH and thyroid hormones, the proper regulation of the thyroid of ewes and lambs was confirmed. The thyroid activity was higher in the spring than in the autumn. The hypothyroidism in the autumn was not proved because no referential values of TSH are set. In the autumn there was a lower level of glucose and cholesterol in the blood of the ewes determined which gives evidence of a lower energy income from the feed in the autumn. Also the urea level which is closely connected to the energy shortage, starvation, and high income of N substances in the feed dosage was in the autumn twice as high as in the spring. There was a positive correlation determined between the higher urea concentration and the higher thyroid activity. Owing to the change of the feed content, the TSH started to stimulate the thyroid to a higher thyroid hormones production and thus to its higher activity.
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Concentrations of thyroid hormones in the blood plasma of sheep
DŘÍZHALOVÁ, Blanka
The theoretical part of the thesis is dealing with description of the thyroidal hormones thyroxine, triiodthironine and their active (unbound) variants in the blood plasma. Thyroid gland, where the hormones are produced, production of very hormones and their effect on whole organism is described here. The practical part tracks and analyzes mutual relations and dynamics of thyroid hormones of sheep in their plasma depending on upraised uptake of iodine and changing uptake of selenium. The results are processed statistically.
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