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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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Virus eradication method by cryopreservation tool - a cryoknife
Bilavčík, A. ; Bobrova, O. ; Faltus, M. ; Fránová, Jana ; Hammond, S. ; Koloniuk, Igor ; Zámečník, J.
Currently, in the nursery production of plant material, emphasis is placed on the propagation of healthy material. For this reason, it is necessary to pay attention to the topic of healthy virus-free nuclear stock when preserving collections of genetic resources of vegetatively propagated plants. Some raspberry viruses are difficult to remove from the plant host by existing methods of thermotherapy or chemotherapy. That is why, using the example of black raspberry necrosis virus (BRNV), a comprehensive methodology for testing health status and cryotherapy in raspberry was developed. The resulting methodical procedure makes it possible to eradicate BRNV from raspberry using cryotherapy of in vitro cultures. The user of the methodology is the Ministry of Agriculture, which applies it as part of the 'National Program for the Conservation and Use of Genetic Resources of Plants, Animals and Microorganisms Important for Nutrition and Agriculture'.
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