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Role of yeast WSS1 protease in DNA repair.
Adámek, Michael ; Grantz Šašková, Klára (advisor) ; Čáp, Michal (referee)
Sustaining the integrity of DNA throughout the lifetime is critical for every living organism. Therefore organisms evolved numerous ways to detect and repair different types of DNA damage caused by various endogenous and exogenous factors resulting in replication stress. Defects in these repair mechanisms can lead to severe human diseases such as neurological disorders, familial cancers or developmental syndromes. In presented master thesis, we investigated the function of a yeast protein named Wss1, a metalloprotease that participates in a recently discovered DNA repair pathway that proteolytically removes DNA-protein crosslinks. Wss1 shows strong negative interaction with another DNA repair protease, Ddi1, in which case was discovered, that double-deleted yeast strain lacking WSS1 and DDI1 is hypersensitive to hydroxyurea. Hydroxurea is a ribonucleotide reductase inhibitor that, in the end, arrests cells in the S-phase of cell-cycle. Based on previous studies, we performed rescue experiments with various deletions and single-site mutants of Wss1p to assess the involvement of particular yeast Wss1p domains in the replication stress response to hudroxyurea.
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The relation between microbial diversity and biodegradation of organic pollutants in soils
Adámek, Michael ; Svobodová, Kateřina (advisor) ; Matyska Lišková, Petra (referee)
This work sums up the knowledge of the currently studied microorganisms capable of degradation of organic compounds contaminating soil and of the impact of biodiversity on biodegradation efficiency. As documented in many studies effective soil remediation can be achieved by mixed-species consortia isolated from polluted soils. However, use of these cultures for bioaugumentation requires further research on their influence on the biodiversity of autochthonic soil microflora. Though bioaugumentation provably affects bioremediation effectivity no ideal carrier for microbes has been found yet that would provide survival of the introduced organisms in the competitive soil environment. Next, selection of suitable bioaugumentation agents should be based on previous analyses of autochthonic microbes in the targeted contaminated soils. Further, this work shows that the presence of key species might be more important for the biodegradation efficiency than biodiversity of soil microflora. Biodiversity is more related to the functionality of soil ecosystems which can be affected by the presence of contaminants leading to positive selection of taxa capable of pollutant degradation. However, there are just a few studies on the relationship between biodiversity and degradation of pollutants. Its further research...
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Role of yeast WSS1 protease in DNA repair.
Adámek, Michael ; Grantz Šašková, Klára (advisor) ; Čáp, Michal (referee)
Sustaining the integrity of DNA throughout the lifetime is critical for every living organism. Therefore organisms evolved numerous ways to detect and repair different types of DNA damage caused by various endogenous and exogenous factors resulting in replication stress. Defects in these repair mechanisms can lead to severe human diseases such as neurological disorders, familial cancers or developmental syndromes. In presented master thesis, we investigated the function of a yeast protein named Wss1, a metalloprotease that participates in a recently discovered DNA repair pathway that proteolytically removes DNA-protein crosslinks. Wss1 shows strong negative interaction with another DNA repair protease, Ddi1, in which case was discovered, that double-deleted yeast strain lacking WSS1 and DDI1 is hypersensitive to hydroxyurea. Hydroxurea is a ribonucleotide reductase inhibitor that, in the end, arrests cells in the S-phase of cell-cycle. Based on previous studies, we performed rescue experiments with various deletions and single-site mutants of Wss1p to assess the involvement of particular yeast Wss1p domains in the replication stress response to hudroxyurea.
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The relation between microbial diversity and biodegradation of organic pollutants in soils
Adámek, Michael ; Svobodová, Kateřina (advisor) ; Matyska Lišková, Petra (referee)
This work sums up the knowledge of the currently studied microorganisms capable of degradation of organic compounds contaminating soil and of the impact of biodiversity on biodegradation efficiency. As documented in many studies effective soil remediation can be achieved by mixed-species consortia isolated from polluted soils. However, use of these cultures for bioaugumentation requires further research on their influence on the biodiversity of autochthonic soil microflora. Though bioaugumentation provably affects bioremediation effectivity no ideal carrier for microbes has been found yet that would provide survival of the introduced organisms in the competitive soil environment. Next, selection of suitable bioaugumentation agents should be based on previous analyses of autochthonic microbes in the targeted contaminated soils. Further, this work shows that the presence of key species might be more important for the biodegradation efficiency than biodiversity of soil microflora. Biodiversity is more related to the functionality of soil ecosystems which can be affected by the presence of contaminants leading to positive selection of taxa capable of pollutant degradation. However, there are just a few studies on the relationship between biodiversity and degradation of pollutants. Its further research...
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