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National Repository of Grey Literature

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Methodology of resistance evaluation of plum, Prunus domesica L., clone C5 to the Plum pox virus and mixed infections with another two viruses Polák, Jaroslav ; Kumar, Jiban ; Jarošová, Jana The methodology encloses the procedure of preparation and ínoculation of transgenic plants of plum with the individual viruses, the maintenance of field orchard, the evaluation of virus symptoms in leaves, serological and electronmicroscopical detection of viruses in leaves of plum trees, determination of relative concentration of PPV by DAS-ELISA, detection of PPV-Rec, ACLSV and PDV by RT-PCR, and determination of risk of recombinations between PPV-Rec and the homologous transgenic transcript in P. domestica, clone C5. Fulltext: PDF Detailed record

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