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Diagnostic of viral hepatitis B in the laboratory of serologic and molecular biology
Vagner, Petr ; Voxová, Barbora (advisor) ; Žáčková, Marta (referee)
Author: Petr Vagner Title: Diagnosis of viral hepatitis B in the serologic and molecular biology laboratory Field of study: Medical laboratory technician, combined type of study The aim of the study: The insight to the diagnosis of viral hepatitis B, with a focus on the examination of antibodies and antigens of Hepatitis B virus. Material and Methods: From 1st January 2011 to 31st December 2013 the serological laboratory of the Institute of Medical Biochemistry and Laboratory Medicine (ÚLBLD) in General University Hospital in Prague (VFN) 77,900 examinations were carried out by chemiluminescent immunoassay system ARCHITECT and system enzyme immunoassay AxSYM, which is used for detection of antibodies and antigens of the virus hepatitis B or detection of molecular biological methods for the detection of hepatitis B virus DNA, the determination of its genotype, drug resistance and pre-core mutations. Results: In total 92.90% of mentioned examinations were serological examinations and 7.10% molecular biological examinations. The examinations of serological markers of HBV were with the highest frequency of HBsAg testing (24, 033 tests) and 16 588 anti-HBs tests, the majority of requests was sent by The Center of Preventive Care (VFN), on the whole 4665 examinations of anti-HBs, from which 3,254 were...
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Diagnostic of viral hepatitis B in the laboratory of serologic and molecular biology
Vagner, Petr ; Voxová, Barbora (advisor) ; Žáčková, Marta (referee)
Author: Petr Vagner Title: Diagnosis of viral hepatitis B in the serologic and molecular biology laboratory Field of study: Medical laboratory technician, combined type of study The aim of the study: The insight to the diagnosis of viral hepatitis B, with a focus on the examination of antibodies and antigens of Hepatitis B virus. Material and Methods: From 1st January 2011 to 31st December 2013 the serological laboratory of the Institute of Medical Biochemistry and Laboratory Medicine (ÚLBLD) in General University Hospital in Prague (VFN) 77,900 examinations were carried out by chemiluminescent immunoassay system ARCHITECT and system enzyme immunoassay AxSYM, which is used for detection of antibodies and antigens of the virus hepatitis B or detection of molecular biological methods for the detection of hepatitis B virus DNA, the determination of its genotype, drug resistance and pre-core mutations. Results: In total 92.90% of mentioned examinations were serological examinations and 7.10% molecular biological examinations. The examinations of serological markers of HBV were with the highest frequency of HBsAg testing (24, 033 tests) and 16 588 anti-HBs tests, the majority of requests was sent by The Center of Preventive Care (VFN), on the whole 4665 examinations of anti-HBs, from which 3,254 were...
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