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Induction and inhibition of acenaphthenol dehydrogenase
Štěrbová, Klára ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Klára Štěrbová Title, Name, Surname of tutor: Doc. RNDr. Lenka Skálová, Ph.D. Title of a diploma work: Induction and inhibition of acenaphthenol dehydrogenase Breast cancer belongs to hormone-dependent tumours. Apart from lifetime exposure to estrogens, the volume of female active sex hormones in breast tissue also plays a big part in the development of this disease. One of mechanisms regulating concentration of estrogens in the target tissue at the pre-receptor level is their interconversion from inactive estron to its active form, 17β-estradiol enzymes from the aldo-ketoreduktas 1C (AKR1C) subfamily. On the basis of specific substrates, inductive and inhibitive studies, enzymes involved in the conversion of individual hormones can be characterized. Relatively specific substrate for AKR1C is acenaphthenol (AcNOH). However, due to the fact that in a multi-enzyme model system the involvement of other enzymes in the reaction cannot be excluded, the enzymes which take part in conversion of acenaphthenol labeled "acenaphtenol dehydrogenase" (AND). The Aim of my diploma paper is to spectrofluorimetrically determine the activity of AND in cytosole taken from breast...
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Induction and inhibition of acenaphthenol dehydrogenase
Štěrbová, Klára ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Klára Štěrbová Title, Name, Surname of tutor: Doc. RNDr. Lenka Skálová, Ph.D. Title of a diploma work: Induction and inhibition of acenaphthenol dehydrogenase Breast cancer belongs to hormone-dependent tumours. Apart from lifetime exposure to estrogens, the volume of female active sex hormones in breast tissue also plays a big part in the development of this disease. One of mechanisms regulating concentration of estrogens in the target tissue at the pre-receptor level is their interconversion from inactive estron to its active form, 17β-estradiol enzymes from the aldo-ketoreduktas 1C (AKR1C) subfamily. On the basis of specific substrates, inductive and inhibitive studies, enzymes involved in the conversion of individual hormones can be characterized. Relatively specific substrate for AKR1C is acenaphthenol (AcNOH). However, due to the fact that in a multi-enzyme model system the involvement of other enzymes in the reaction cannot be excluded, the enzymes which take part in conversion of acenaphthenol labeled "acenaphtenol dehydrogenase" (AND). The Aim of my diploma paper is to spectrofluorimetrically determine the activity of AND in cytosole taken from breast...
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Flubendazole Influence on Breast Cells Proliferation and Activity of Acenaphthenol Dehydrogenases
Štěrbová, Klára ; Skálová, Lenka (advisor) ; Lamka, Jiří (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Title, Name, Surname of candidate: Mgr. Klára Štěrbová Title, Name, Surname of tutor: Prof. RNDr. Lenka Skálová, Ph.D. Title of a thesis: Flubendazole influence on breast cells proliferation and activity of acenaphthenol dehydrogenases This thesis investigates the effect of flubendazole (FLU) on the proliferation of breast cancer cell lines MCF-7 and MDA-MB-231 and nonmalignant breast line MCF-10A. The anthelmintic drug FLU is widely used for treatment cestodoses and nematodoses. The mechanism of FLU's effect against nematodes and cestodes is disruption of the cytoplasmic system of microtubules. In 2010 it was found out that FLU disrupts the microtubules of tumor cells and significantly inhibits the proliferation of leukemia and myeloma cells. The main aim of this paper was to determine whether FLU also inhibits proliferation of breast cancer cells. Another objective was to determine whether FLU interferes with the synthesis of estradiol, because this active estrogen plays an important role in the development of breast cancer. The proliferation of breast cancer cells and nonmalignant cells was usually tested by two viability tests (MTT and NRU) and by continuous monitoring impedance using...
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