|
|
Recognition of microbial patterns in earthworms
Škanta, František ; Bilej, Martin (advisor) ; Kopečný, Jan (referee) ; Šimek, Miloslav (referee)
Survival of earthworms in the environment depends on their ability to recognize and eliminate potential pathogens. Two closely related earthworm species Eisenia andrei and Eisenia fetida inhabit different environment with specific microbiota. Both species can be reliably determined using of species-specific primers for cytochrome c oxidase I (COI) and stringent PCR conditions. Whereas, we did not observed any substantial differences in the expression and activity of CCF and lysozyme upon microbial challenge, the expression as well as the hemolytic activity of fetidin/lysenins was considerably higher in E. andrei as compared to E. fetida. Genomic DNA analyses revealed significantly higher level of fetidin/lysenins in E. andrei compared to E. fetida suggesting hypothetical gene duplication. Earthworms live in permanent close contact with microbial environment. Coelom cavity as well as the gut of E. andrei earthvworm differs in the number of bacteria. The number of bacteria in the gut is more than six time higher than in coelomic fluid. High microbial load of E. coli O55, B. subtilis W23, and S. cerevisiae S288 in the earthworm environment, resulted in an increase of microorganisms in both, the coelom and the gut. The changes in mRNA levels of defense molecules (pattern recognition receptors CCF,...
|
guest ::
RSS feed.