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Contaminants in dust and risks to human health
Šafaříková, Linda ; Erban, Tomáš (advisor) ; Hubert, Jan (referee)
This bachelor's thesis addresses the impact of contaminant occurrence in dust and how these contaminants can affect human health. The focus is primarily on allergens and potential allergens. The theoretical part introduces major allergens and methods for their separation and identification. Identifying the spectrum of allergens in the environment is crucial for discerning the causes of allergic reactions. Some methods based on antibody detection, such as Western blot or ELISA, enable the identification of specific individual allergens in samples. In cases where antibodies are not available, alternative approaches are necessary. An appropriate approach is proteomic analysis, which holds significant potential for identifying the entire spectrum of allergens and other proteins in dust. Especially, high-capacity proteomic approaches enable the identification and characterization of multiple proteins, including allergens, simultaneously, providing a comprehensive view of dust sample composition. In the practical part, high-capacity proteomic methods were employed. A combination of gel-based and gel-free analysis of overall heterogeneous dust samples and band analysis after 1D-E separation was used for household dust analysis. Samples were collected from beneath the bed and behind the wardrobe, where the...
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