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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
In planta production of TMV (Tobacco mosaic virus) nanoparticles of specific length
 Dlabalová, Lucie ; Moravec, Tomáš (advisor) ; Šafářová, Dana (referee)
Tobacco mosaic virus (TMV) is one of the most investigated viruses and its attributes and structure are therefore well-known. In this work, we have chosen TMV as a biotemplate for the adjustable-length particles production in plants. The viral RNA and coat protein of TMV self-assemble into particles under physiological conditions. The particle length depends on the length of packaged RNA. The encapsidation signal that is necessary for preferential viral RNA packaging by coat protein disks is known and characterized since the 1980's. In this work, we have proposed a two-component system based on a Nicotiana bentamiana plants infection with packaging competent defective RNA (dRNA) and a helper virus RNA which provides all the components necessary for dRNA replication and packaging. The encapsidation signal in the helper virus sequence was removed to avoid formation of particles of incorrect length. Some of our helper viruses contained a coat protein with modified region of the particle's inner channel. This modification should allow specific binding of metal atoms within the core of the rod shaped particle. Several variants of dRNA and helper viruses were prepared to identify individual areas important for the replication, encapsidation and nanoparticle stability. We focused on the particle formation...
Detailed record
In planta production of TMV (Tobacco mosaic virus) nanoparticles of specific length
 Dlabalová, Lucie ; Moravec, Tomáš (advisor) ; Šafářová, Dana (referee)
Tobacco mosaic virus (TMV) is one of the most investigated viruses and its attributes and structure are therefore well-known. In this work, we have chosen TMV as a biotemplate for the adjustable-length particles production in plants. The viral RNA and coat protein of TMV self-assemble into particles under physiological conditions. The particle length depends on the length of packaged RNA. The encapsidation signal that is necessary for preferential viral RNA packaging by coat protein disks is known and characterized since the 1980's. In this work, we have proposed a two-component system based on a Nicotiana bentamiana plants infection with packaging competent defective RNA (dRNA) and a helper virus RNA which provides all the components necessary for dRNA replication and packaging. The encapsidation signal in the helper virus sequence was removed to avoid formation of particles of incorrect length. Some of our helper viruses contained a coat protein with modified region of the particle's inner channel. This modification should allow specific binding of metal atoms within the core of the rod shaped particle. Several variants of dRNA and helper viruses were prepared to identify individual areas important for the replication, encapsidation and nanoparticle stability. We focused on the particle formation...
Detailed record

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3 ŠAFÁŘOVÁ, Denisa
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