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National Repository of Grey Literature

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Průzkum výskytu oportunního prvoka, \kur{Giardia intestinalis}, u zdravých lidí a jejich zvířat v ČR s použitím qPCR BROŽOVÁ, Kristýna The main aim of this study was to optimize the qPCR diagnostic protocol for determination of the prevalence of intestinal protist, Giardia intestinalis, in a volunteer group across the Czech Republic. A total of 428 samples were collected from gut-healthy humans (296) and from animals (132) with which these people were in close contact. The overall prevalence of G. intestinalis was 7 % in humans and 19 % in animals. In addition, we compared the sensitivity of two molecular methods for detecting of the presence of giardia, specifically qPCR and conventional PCR. Based on our results, we found out that qPCR is more sensitive method than PCR. Detailed record

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