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New aproaches to myocardial atrophy attenuation after mechanical unloading - Experimental study of heterotopic heart transplantation in rats
Pokorný, Martin ; Malý, Jiří (advisor) ; Ošťádal, Petr (referee) ; Rohn, Vilém (referee)
Cardiac atrophy, as a result of a long term mechanical unloading observed in patients treated with mechanical circulatory supports, represents a substantial obstacle in cardiac recovery. The first aim of this thesis was to evaluate the course of unloading-induced cardiac atrophy in healthy and failing heart. Mechanical unloading was studied in an experimental model of heterotopic heart transplantation in rats. Heart failure was experimentally induced by chronic volume overload achieved by creation of aorto-caval fistula. The second aim of this thesis was to discover if dietary induced increase in fatty acids concentration in heart tissue, specifically myristic, palmitic and palmitooleic, that are believed to promote physiological heart growth, could attenuate the course of cardiac atrophy. The third aim of this thesis was to examine if increased isovolumic loading obtained by intraventricular implantation of newly designed spring expander would attenuate the development of cardiac atrophy. The level of cardiac atrophy was evaluated as the weight loss of the heterotopically tranplanted heart compared to the control heart on days 7, 14, 21 and 28 after heterotopic transplantation. The cardiomyocyte width and the level of cardiac fibrosis was studied simultaneously. Mechanical unloading following heterotopic...
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