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Neuroprotective effects of food intake regulating peptides in vitro and in vivo
Kasperová, Barbora Judita ; Maletínská, Lenka (advisor) ; Vaněk, Ondřej (referee)
Nowadays, Alzheimer's disease (AD) is one of the most serious health problems among elder. To this day, pathogenesis of AD is still unknown and therefore no effective treatment has been found. AD is characterized by neuropathological features, the formation of extracellular senile plaques of amyloid β and intracellular neurofibrillary tangles of Tau protein. Numerous experimental studies have confirmed that metabolic disorders such as type 2 diabetes mellitus or obesity contribute significantly to the development of cognitive impairment and therefore the development of AD. In this diploma thesis, the potential neuroprotective effect of peptides regulating food intake was investigated in in vitro and in vivo experiments. The potential neuroprotective effect of liraglutide, a glucagon-like peptide-1 analog used as a type 2 diabetes mellitus treatment, a prolactin-releasing peptide (PrRP) and its palmitoylated analog, palm11 -PrRP31, was investigated on SH-SY5Y cell line. The peptides were used as a pretreatment on SH-SY5Y cells in methylglyoxal-induced cytotoxicity. It has been proven that the peptides themselves are not toxic and do not significantly reduce the viability of SH-SY5Y cells. The tested peptides showed prophylactic effects against cytotoxicity and apoptosis induced by toxic...
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