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National Repository of Grey Literature

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Setting of Optimal Algorithm for Effective Diagnostics of Non-Small Cell Lung Carcinoma with ALK Gene Rearrangement Rozkoš, Tomáš ; Ryška, Aleš (advisor) ; Dundr, Pavel (referee) ; Zámečník, Josef (referee) (Identification of optimal algorithm for effective diagnostics of non-small cell lung carcinoma with ALK gene rearrangement - implementation of the method and practical experience with routine diagnostics) INTRODUCTION: Smaller subgroup of non-small cell lung carcinoma (NSCLC) is represented by tumours with carcinogenesis iniciated by fusion of ALK gene with another partner (usually EML4). Patients with ALK gene rearrangement treated with ALK inhibitors have significantly prolonged survival. Since ALK gene rearrangement is described according to the current WHO classification in 4-5% of NSCLC, it is necessary to find the optimal way of identifying patients eligible for this targeted therapy in routine diagnostic practice. AIM: In the retrospective part of the study a) to find an immunohistochemical (IHC) detection procedure of ALK protein with specificity and sensitivity high enough to use this antibody as screening method for selecting NSCLC cases for fluorescence in situ hybridization (FISH) testing of ALK gene rearrangement and b) to determine diagnostic yield of "small" biopsies, i.e. endobronchial, transbronchial and transthoracic biopsies and cytoblocks, for ALK gene rearrangement testing. In the prospective part of the study a) verification of the selected IHC method of ALK protein detection... Detailed record

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