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Effect of the availability of iron compounds on the expression of molecules involved in the transport of non-transferrin iron ions: In vitro study concerning human cell lines K562 and Caco-2
 Balušíková, Kamila
Iron absorption, transport, and storage in the body is very strictly regulated mechanism by the reason of absence of a controlled pathway provided its excretion. Hence we are interested in transport mechanism of non-haem iron which is mediated by molecules DMT1 (divalent metal transporter 1, membrane iron importer), Dcytb (duodenal cytochrom b, membrane ferrireductase), ferroportin 1 (membrane iron exporter), hephaestin (membrane ferroxidase) and ceruloplasmin (cytoplasmatic ferroxidase) and enable iron uptake from food up to its binding to plasma transferrin. Our project monitors the effect of iron availability to the expression of the molecules potentially involved in non-transferrin iron transport across cell membranes. We studied the influence of iron deficiency and iron overload to the regulation of iron uptake by these molecules in various functional types of human cells. Cells were maintained in RPMI 1640 medium and supplemented with other additives. The expression was tested on mRNA level by quantitative real-time PCR with reverse transcription in in vitro study using human cell lines K562 and Caco-2. K562 cells (human erythroleukemia) represent cells with high utilization of no-transferrin iron and Caco-2 cells (human colorectal carcinoma) is a model of cells with different apical and...
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