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Posouzení subchronické toxicity směsí pesticidů na raky
HOVORKOVÁ, Anna
Prometryn, belonging to the group of s-triazines, has been extensively used in the case of protection of maize production against broadleaf weeds and grasses. Its use was banned by the EU in 2004. Chloridazone is a pyridazone herbicide used to protect beet production and to control broadleaf weeds. Its properties increase the potential for the transfer of this pesticide to the aquatic environment. Together with its metabolites, it has been detected in groundwater in the Czech Republic. Its use in the Czech Republic has been banned since 2020. The adventitious properties and long-term use of these substances may increase the risk of contamination of non-target ecosystems by the pesticides used and their metabolites, which may form mixtures in this environment and thus have an even riskier impact on the environment. The aim of this work was to assess the subchronic toxicity of the pesticide mixture formed of the concentrations of these pesticides on the signal crayfish (Pacifastacus leniusculus) in comparison with the control and pesticides tested alone. A subchronic toxicity test of 20 days duration followed by 10 days depuration was used to obtain the results. The criteria evaluating the toxicity of the mixture were the biochemical profile of haemolymph, oxidative stress level and antioxidant enzyme activity from hepatopancreas, muscle and gill samples. The biochemical profile of haemolymph was significantly affected in individuals tested in the pesticide mixture. For biomarkers altered due to the mixture, suppression of the effect of prometryn in the mixture was observed in some cases. The analysis of antioxidant biomarkers did not show a higher toxicity of the mixture over the pesticides tested alone. In the case of antioxidants, a greater effect was observed for prometryn tested alone, where in some cases its presence in the mixture suppressed the effects of chloridazon in the mixture.
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Non-fish prey in the diet of fish-eating mammals, Eurasian otter (Lutra lutra) and mink (Neovison vison)
Tuzarová, Veronika ; Čech, Martin (advisor) ; Poledníková, Kateřina (referee)
The Eurasian otter (Lutra lutra) is considered, along with the American mink (Neovison vison), a significant fish predator not only in the Czech Republic but also in other regions across Europe. One of the goals of this study is to determine, based on available literature, the types of prey that form the diet of the Eurasian otter and the American mink, specifically focusing on the non-fish component of their food and how the percentage representation of these items changes in the diet of both species depending on habitat and season. Another objective is to verify whether the American mink has a higher proportion of non-fish prey in its diet compared to the Eurasian otter. The processed data describing the diet through spraint analysis in biomass units were divided according to climatic regions, i.e., temperate, Mediterranean, and also based on the type of aquatic environment, i.e., stagnant or flowing water. The results of the study indicate that the American mink consumed, on average, three times more non-fish prey (74 % biomass) throughout the year than the Eurasian otter (24 % biomass). The seasonal variation had the most notable impact on the consumption rate of frogs and birds in the non-fish diet of both predators. According to the analyzed data, both predators especially focused on frogs,...
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Monitoring reakce raků na různé podněty: jsou raci dobrými bioindikátory?
MALINOVSKA, Viktoriia
Freshwater crayfish play a vital role in food webs and energy transfer between trophic levels, often acting as keystone species. Crayfish are prime species among invertebrates, considering their large size, abundance, and biodiversity. Crayfish belong to common model organisms in scientific research and have been used as bioindicators of water quality in laboratory and field studies. Freshwater crayfish are at direct risk while facing polluted aquatic environments, as many chemicals enter natural waters throughout the year. Studies have been shown that crayfish exhibit a variety of behavioral and physiological alterations in response to anthropogenic contaminants. In Chapter 2, we aimed to assess the cardiac and locomotor responses of crayfish Pacifastacus leniusculus to short-term pulse exposure to environmentally relevant concentrations of three pesticides (metazachlor, terbuthylazine, and thiacloprid). A significant increase in heart rate and distance moved was observed in metazachlor-exposed specimens compared to controls. We did not detect significant changes in crayfish cardiac and locomotor activity in terbuthylazine and thiacloprid groups. Most crayfish in these groups did not exhibit locomotor response, so they might not be stimulated to escape a contaminated area. This may lead to continuous exposure to environmental chemicals that are potentially harmful. In Chapter 3, we investigated the effects of UV filters Benzoyl-4-hydroxy-2-methoxybenzenesulfonic acid (BP4) and 2-Phenylbenzimidazole-5-sulfonic acid (PBSA) on crayfish during 30 min exposure period. Specimens exposed to the tested compounds moved greater distances and spent more time in locomotion. Increased heart rate was also detected in treated groups. Such behavioral and physiological alterations of crayfish detected in both studies can potentially impair crayfish ability to respond to predators, and consequently affect abundance and the functional properties of macroinvertebrate communities. Along with use of crayfish in laboratory and field experiments, there is also an option to conduct case study with crayfish acting as bioindicators. We evaluated crayfish physiological responses to water disinfected with chlorine dioxide in long-term monitoring under operating conditions in local brewery (Chapter 4). The disruption of cardiac rhythmicity was detected with chaotic increases and decreases of heart rate regardless of the time of day. In natural environment, heart rate of crayfish is higher during night time considering nocturnal activity of these decapods. Nocturnalism is important adaptation for crayfish, and its impairment can lead to certain risks related to increased visibility to predators and alterations in foraging. Our results indicated that crayfish exhibit a cardiac and locomotor responses when shortly exposed to commonly used herbicide metazachlor and two widely applied UV filters BP4 and PBSA, suggesting that crayfish can potentially detect the appearance of these aquatic contaminants in water. The usefulness of crayfish in long-term monitoring of water quality have also been demonstrated. These findings expand our understanding of using crayfish as bioindicators in scientific research. In conclusion, this thesis provides the data from laboratory and case studies focusing on measurements of cardiac and locomotor endpoints in crayfish. Given that crayfish is at the base of the food chain in freshwater environments, it is of great importance to study the effects of different stimuli on such keystone species. Understanding further biological impacts of aquatic pollutants on freshwater crayfish and other non-target taxa will benefit our knowledge of threats to functional compositional changes in populations and communities.
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Aktivita raka signálního v podmínkách malého toku
DAVID, Jaroslav
The signal crayfish is one of the oldest invasive crayfish species in Europe, causing ecological and often economic damage in newly invaded locations. The invasion and associated problems can be monitored in real-time on the Křesanovský brook, where regular eradication measures have been carried out for six years. However, the detailed and comprehensive impact of these activities on the crayfish population as a whole and any changes in individual behaviour have not yet been studied in detail. To obtain relevant data for this purpose, this thesis video-recorded the situation in the stream in monthly cycles from May to September 2021, with recordings taken the night before and the night after thorough manual crayfish catching in the brook, evaluating the number and activity of observed crayfish. The results did not show that the trapping had changed the overall crayfish activity. Even with a high number of captured crayfish, the weakened crayfish population after trapping showed the same overall activity as the complete population before trapping (presumably due to an increase in individual activity in response to catching and accompanied habitat disturbance). Furthermore, the results underline the previously assumed low success rate of eradication efforts at the site and confirm that barriers in the stream are not sufficient to stop the signal crayfish spread. These findings will help to better understand the impact of eradication measures on signal crayfish populations. As this was the first research of its kind, this thesis opens up opportunities for methodological improvements and further development, as well as posing many new questions that need to be addressed. The ultimate goal of these efforts is to improve management interventions against invasive signal crayfish.
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Sublethal effects of pesticide mixtures on crayfish
HOVORKOVÁ, Anna
Prometryn, a triazine herbicide, was banned in Europe in 2004. Metazachlor, a chloracetamide used to treat mainly oilseed crops, is one of the herbicides still in use. Individual pesticides or their metabolites can cause adverse effects in the aquatic environment, and the resulting mixtures of pesticides that can form in the environment are another issue in terms of adverse effects in the environment. The aim of this study was to assess the effect of mixtures of pesticides mixed from selected concentrations (0.01 mg.l-1, 0.1 mg.l-1, 1 mg.l-1). Pesticides were also tested individually at selected concentrations. The biochemical profile of hemolymph and antioxidant biomarkers and oxidative stress biomarker in hepatopancreatic, muscle and gill tissues of marbled crayfish were determined. Toxicity was assessed using a subchronic toxicity test performed over a period of 8 days. Metazachlor concentrations (0.1 and 1 mg.l-1) caused decreasing ALT, Cl-, Mg2+ and Ca2+ levels and increasing NH3 levels in hemolymph. The effect of all tested mixtures was observed on the increase of GLO, ALT and NH3 levels, here a synergistic effect of the mixtures was observed. The activity of antioxidant biomarkers was affected only in CAT for prometryn (0.01 mg.l-1) and metazachlor (0.01 and 1 mg.l-1). LPO levels were affected by metazachlor (0.01 mg.l-1). There was no effect of pesticide mixtures on LPO levels and antioxidant enzyme activities.
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Pach vítězství: role feromonů v interakcích raků
MAREŠ, Josef
Analysis of communication, hierarchy, and role of pheromones in crayfish is an interesting area that constantly brings new knowledge and can help us understand some facts that may be valid not only for crayfish but also other crustaceans. In this work, it was therefore studied how the offspring of two females behave in a one tank. After the dominance establishment in females (after a series of contacts and fights), the offspring of preferred a dominant female only, regardless of if their own or a unfamiliar mother. The dominant female, that successfully fought the fights, collected juveniles by its active movement, and they followed its movements. The subordinate female accepted her position, tried to avoid contacts, and reduced its activity. The juveniles practically ignored the subordinate mother at this stage of the experiment. Within the diploma thesis, all set goals were achieved and the newly discovered facts may be an important part of further follow-up experiments. We were able to respond positively to all primary hypotheses when: 1 / Females of marbled crayfish in one tank always established a relationship of the dominant and subordinate one, 2 / marbled crayfish juveniles always chose only one mother in a common tank, and 3 / it was in all cases the dominant one. The results show that dominance (whether communicated chemically or by visual stimuli) is a more important factor for juvenile crayfish than the specific brood pheromeone of their mother. The results bring significant knowledge to the issue of the hierarchy of various chemical and visual signals in crayfish communication. Thus, certain communication signals seem to be superior to others. The "winner odours" are probably stronger than such a strong stimulus as the brood pheromone responsible for recognizing the offspring and the mother. On the other hand, it is not possible to rule out a possible effect of the unattractiveness of the subordinate mother. How the relationships among the subordinate female, dominant female and their offspring would develop in remains opened to further research hypotheses.
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Porovnání přežívání a růstu raka červeného a raka mramorovaného v teplotně suboptimálních podmínkách
DAVID, Jaroslav
Biological invasions are increasingly recognized as biological and economic threats. The numbers of these invasions continue to rise over time, disrupting the original biodiversity. In this experiment, we focused on two species of crayfish native to North America, the red swamp crayfish and the marbled crayfish, which are among the most important invasive crayfish worldwide. To determine which of these species should be dominant in permanently suboptimal temperature conditions (water temperature of 16 °C), we conducted an experiment evaluating growth, mortality, claws damage, and maturation in single-species and mixed stocks. It turned out that the faster-growing species was the red swamp crayfish, which grew the fastest in mixed stocks, at the expense of marbled crayfish, which, on the contrary, grew more slowly in these stocks than in single-species stocks. There were no large differences in survival in the individual groups, except single-species marbled crayfish stocks, for which the survival rate was about 15% higher than in other groups. The incidence of individuals with damaged claws was higher for red swamp crayfish, a large difference was recorded between marble crayfish groups, when in mixed stocks the damage was circa five times higher contrary to the single-species setup. At the end of the experiment (week 18), three mature males with gonopods and one female with glair glands were found in the red swamp crayfish.
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Kdo je boss teď? aneb dopad farmaka sertralinu na hierarchii raků
MAREŠ, Josef
Pharmaceuticals' pollution of open waters is a current threat to aquatic organisms. The development of pharmacies and increased consumption of drugs leads to a burden on the aquatic environment with dangerous pollutants. Due to the fast and stressful lifestyle, the consumption of antidepressants increases significantly in the majority of the human population. After consumption and excretion, antidepressants and other drugs enter groundwater and surface water through wastewater treatment plants, which can have a secondary effect on non-target organisms. This master thesis assesses the possible impact of the commonly used antidepressant sertraline on the mutual social behavior of marbled crayfish (Procambarus virginalis). The experiment was based on monitoring the behavior of different pairs of different crayfish. The created dyads assessed mutual interactions between control crayfish, crayfish exposed to sertraline, and reciprocally between both crayfish groups. Three weeks before monitoring, crayfish were exposed to an environmentally relevant sertraline concentration of 1 g?l-1. The results were evaluated by sensory assessment according to available methods, and subsequently, the statistics were assessed. No group of crayfish observed showed a significant difference in behavior. Thanks to the initial phase of experiments of this type, we cannot determine with certainty from the results that sertraline does not affect the social behavior of marbled crayfish. However, along with other antidepressants, sertraline, a selective serotonin reuptake inhibitor, is undoubtedly a potential threat to the environment.
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Vliv chloridazonu na raná vývojová stádia raka mramorovaného
ŘEHOŘEK, Martin
The main objective of this bachelor thesis is to evaluate the influence of chloridazon on the early growing stages of marbled crayfish (Procambarus virginalis). The influence of chloridazon is assessed using various factors such as biomarkers of oxidative stress, growth, mortality, ontogenetic development, and crayfish behaviour. The acquired results from this research will extend the current knowledge and data on the influence of chloridazon on crayfish and on water ecosystems.
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Vliv chronické expozice chloridazonu a jeho metabolitu chloridazon desphenylu na raka signálního
MATERNA, Jan
The aim of this thesis was to evaluate the effect of chloridazon (Ch) and its major metabolite chloridazon desphenyl (Ch-D) at environmentally relevant concentrations 0.45 micrograms/l (Ch1; Ch-D1) and 2.7 micrograms/l (Ch2; Ch-D2) on adult signal crayfish (Pacifastacus leniusculus). The exposure was 30-day and followed by a 15-day depuration period. The effect of the tested substances was evaluated on the basis of behavior, biochemical profile of hemolymph, biomarker of oxidative stress and antioxidants parameters. The tested substances did not effect on the behavior of crayfish. Chloridazone at concentration 2.7 micrograms/l (Ch2) caused a significant (P<0.01) increase of glucose (GLU), lactate (LACT), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in haemolymph of crayfish and changes in catalase (CAT) levels in the gills and the hepatopancreas, compared to controls. Crayfish exposed to chloridazon in both tested concentrations (0.45 micrograms/l and 2.7 micrograms/l) showed significantly (P<0.01) higher level of reduced glutathione (GSH) in hepatopancreas and gills compared to control. Exposure of chloridazon desphenyl at concentration 0.45 micrograms/l and 2.7 micrograms/l caused significantly (P<0.01) higher levels of glucose, lactate, ammonia (NH3), calcium (Ca), alanine aminotransferase and aspartate aminotransferase activity in haemolymph of exposed crayfish compared to control. Chloridazon desphenyl in both tested concentrations caused significant (P<0.01) changes in the levels of lipid peroxidation (LPO) in the hepatopancreas and catalase (CAT) and GSH level in the gills and hepatopancreas compared to the control group. Some effects persist after a depuration period. The results showed that the metabolite chloridazon desphenyl has more pronounced effects on the physiology of adult signal crayfish than its parent compound chloridazon. It can be stated, that chloridazon and its metabolite chloridazon desphenyl has the strongly negative and harmful effects of on crayfish as representatives of non-target aquatic organisms.
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