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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Vliv adipokinetického hormonu na působení jedu z vosičky, \kur{Habrobracon hebetor}, ve svalové tkáni octomilky, \kur{Drosophila melanogaster}
 PODLAHOVÁ, Šárka
Adipokinetic hormone (AKH) delays or reduces neuromuscular paralysis caused by the venom of the parasitic wasp Habrobracon hebetor, however the associated mechanism is not known yet. This thesis describes in detail the technique of preparation of semithin and ultrathin sections analyzed by a transmission electron microscopy that enabled detailed observation of changes in muscular ultrastructure of the Drosophila melanogaster thorax after the wasp venom and AKH treatments. The results revealed that the wasp venom induced significant changes in the ultrastructure of myofibrils and mitochondria; however, the changes were reduced after co-application with AKH. AKH itself did not affect organization of muscle fibres but elicited vacuolization in mitochondria. Furthermore the wasp venom treatment led to significant prolongation of sarcomeres.
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Příprava rekombinantního paralyzačního proteinu z jedu parazitoidní vosičky \kur{Habrobracon hebetor}
 MARTÍNKOVÁ, Barbora
A candidate protein from the venom gland of parasitoid wasp, Habrobracon hebetor, predicted to be responsible for the paralysis of lepidopteran caterpillars, was produced in baculovirus and bacterial expression systems. The function of both recombinant protein variants was confirmed by in vivo tests in Galleria mellonella larvae.
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