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National Repository of Grey Literature

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Membrane potential measurement with voltage sensitive dyes in confocal microscopy Heczková, Monika ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor) The aim of this work is to make a literature search in the measurement of membrane voltage using the voltage-sensitive dyes and suggest a method of measuring the membrane voltage on the available cells using the voltage-sensitive dye Di-4-ANEPPS and RH237. The work contains an introduction to electrophysiology of cell, explains fluorescence and typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document was largely devoted to characterization and distribution of voltage-sensitive dyes. The output of a design solution is a real experiment. Detailed record

Membrane potential measurement with voltage sensitive dyes in confocal microscopy Heczková, Monika ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor) The aim of this work is to make a literature search in the measurement of membrane voltage using the voltage-sensitive dyes and suggest a method of measuring the membrane voltage on the available cells using the voltage-sensitive dye Di-4-ANEPPS and RH237. The work contains an introduction to electrophysiology of cell, explains fluorescence and typical fluorescence characteristics. The thesis contains the description of a fluorescence microscope. The document was largely devoted to characterization and distribution of voltage-sensitive dyes. The output of a design solution is a real experiment. Detailed record

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