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Possible Uses of Camp in Interpretation of Czech Contemporary Cultural Background
DEMETER, Peter
Proposed dissertation is focusing on specific eccentric aesthetics based on hyperboles, ambivalent meanings and ironic attitude, which in Anglo-American background is so called camp derived from French. Camp origin can be found on the court of Louis XIV, where we can notice passionate tries to cultivate affected court-style culminating in baroque metaphor of the world as a theatre. Due to impact of modernity and postmodernity, scope of the camp extended to various cultural spheres and phenomenon began to corelate through its eccentricity with common marginality, respectively with something what is different from "normality" and "genuineness" also settled schemes of perception or ways of historical reflection. Goal of proposed dissertation is based on theoretical-historical interpretations to trace mentions of camp (mainly in literature) with focus on Czech background, where its rich interpretational potential can clarify most of social-cultural tendencies.
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Vnímavost kaprovitých a nekaprovitých druhů ryb k CyHV-3
POSPÍCHAL, Aleš
Cyprinid herpesvirus 3 (CyHV-3) also known as koi herpesvirus (KHV) is a causative agent of highly contagious disease (koi herpesvirus disease) and can cause significant losses in fish stocks. The disease is restricted to koi and common carp, but recent investigations have shown that other cyprinids as well as non-cyprinid species may be asymptomatically susceptible to this virus and might play roles as potential carriers or can contribute to biological conservation leading to persistence of this virus in environment. Therefore, it seems to be important to verify not only the susceptibility of other cyprinid and non-cyprinid species, but also their ability to transmit KHV infection to susceptible species. We investigated the susceptibility of stone loach (Barbatula barbatula) and sterbel - a hybrid between sterlet (Acipenser ruthenus) and beluga (Huso huso) to KHV. The investigation was performed by means of their cohabitation together with na?ve koi and intraperitoneally KHV-infected koi (primary challenge). This part of investigation is followed by secondary challenge, when a portion of the surviving stone loach and sterbel was cohabited with health na?ve koi (testing of ability to carry KHV). All samples of fish both from primary challenge and secondary challenge were tested for the presence of KHV DNA by nested PCR. In the primary challenge, results of PCR revealed the presence of KHV DNA in 95% of cohabited na?ve koi samples. Furthermore, PCR analysis of fish samples surviving primary challenge revealed the presence of viral DNA in 77.8% (7/9) of stone loach and in 22.2% (2/9) of sterbel. In case of samples of fish coming from secondary challenge, nested PCR did not reveal any of them to be positive for KHV DNA. Next investigation was focused on assessment of the susceptibility of topmouth gudgeon (Pseudorasbora parva). In this case, we performed cohabitation based on two different conditions. All experiments consisted of primary and secondary challenges as well as in all previous cases. Firstly, we tested topmouth gudgeon under standard conditions (no-stress experiment). After the primary challenge, nested PCR did not reveal the presence of KHV DNA in any specimen of cohabited topmouth gudgeon, but all specimens of dead koi were KHV DNA positive. Nested PCR of fish tissues subjected to the secondary challenge did not show the transfer of virus to naive fish. After that, we changed the experimental conditions and we applied two stress factors (scaring by net and removal of skin mucus) to imitate the stress most commonly encountered in the wild. In this case, all samples were tested for the presence of KHV DNA using real-time PCR. After exposure to stress (removal of skin mucus), real-time PCR revealed four out of five samples (80%) of topmouth gudgeon to be positive for KHV DNA. Two out of five samples (40%) of topmouth gudgeon treated by scaring were found to be positive for the presence of viral DNA. Real-time PCR after the secondary challenge did not reveal any viral DNA positivity in specimens of topmouth gudgeon from groups previously exposed to stress. The stress experiments showed that removal of skin mucus might potentially lead to susceptibility of topmouth gudgeon to CyHV-3 infection, but the transmission of the virus to koi carp was not observed. Even though PCRs positive findings of KHV DNA in tissues of fish were relatively low, the presented results of cohabitation assays of cyprinid and non-cyprinid fish species indicate other species showing slight asymptomatic susceptibility to CyHV-3. On the other hand, on the base of our results coming from "virus-carrier" assays, we could not prove that hybrids between sterlet and beluga, stone loach and topmouth gudgeon can transfer this virus to naive koi.
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Genes participating in response to Leishmania major revealed by targeted mutation.
Ezrová, Zuzana ; Lipoldová, Marie (advisor) ; Krulová, Magdaléna (referee)
Leishmania major is an intracellular parasite which often successfully multiplies and disseminates in a body of the host thanks to strategies that help it to escape the components of immune system of the host organism. Thus, the parasites evoke an impairment of regulatory pathways that in physiological conditions lead to an expression of genes involved in a response to L. major and its efficient elimination. Gene targeted deletion, also called gene knock-out, can result in phenotypic alteration and associated enhanced susceptibility or resistance of the host. Although such detected genes do not have to signify their variability in population and hence they may not be responsible for the worsened outcome of leishmaniasis of some people necessarily, studies in which they are analysed and general knowledge being also a subject of this thesis help us together with techniques of forward genetics to reveal the biochemical pathways during the infection and their elements that influence the outcome of the disease and might be useful for researches of new medicine drugs or gene therapy.
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Vnímavost vybraných hostitelů k infekci \kur{Cryptosporidium avium}
REIFOVÁ, Zuzana
Experimental infections with Cryptosporidium avium were carried in three different species of birds (chicken, duck and pheasant) divided into three age categories (1-day-old, 21-day-old and adult). The birds were infected peroraly using intragastric gavage with a dose of 380 thousand of oocysts in 1 ml. Faecal samples were collected daily for 30 days for a microscopic and molecular examination. While all age categories of pheasant (Phasianus colchicus) were not susceptible to C. avium infection, all age groups of domestic fowl (Gallus gallus f. domestica) and white ducks (Anas platyrhynchos f. domestica) were susceptible. Prepatent period in domestic fowl ranged from 9 to 11 days depending on the age category, in white ducks from 4 to 6 days. Patent period for both susceptible host species was longer than 30 days. Based on the results of experimental infections domestic fowl and white duck are proposed as a suitable model hosts for Cryptosporidium avium.
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Aesthetics of Camp
NOVOTNÁ, Pavlína
This thesis deals with the phenomenon of camp and its manifestations in modern western culture. The aim of this thesis is to indentify the signifiant features typical for camp as an aesthetic stance and for camp as an object. Attention is paid to the relationship of the camp and kitsch, and their differences and similarities. The relationship of camp to the high and low art or dandyism is also examined. The main text used for this thesis is an essay "Notes on Camp" by Susan Sontag.
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