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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Introduction of the Histoscanning for Routine Evaluation of the Biopsy of the Native and Transplantated Kidneys.
 ŠMOLÍKOVÁ, Petra
Aim: Immunofluorescence used as part of diagnostic algorithm for native and transplanted kidneys, however it has several limitations: short expiration, difficult handling and shipment. We compared quality of real slides versus scanned digital( virtual)slides. Materiál and methods: 163 cases were stained and diagnosed using classic technology and microscope. All cases were scanned using 3D Histech and evaluated again as virtual slides. Results: 20 primary evaluated slides( real and virtual) were reevaluated. Based on results, changes of technology were introduced ( medium, coverslips, scanning parameters). 143 new cases were examined using classic technology and subsequently using virtual microscopy. Results and minor differences in quality were acceptable and modified technology was introduced into the routine practice. Conclusions: Virtual microscopy is applicable also to immunofluorescence techniques and it is routinely used in our institution now. Altogether with histoscanning of standard histochemical and immunohistochemical stainings is also used for so-called telepathology. Main advantage is easy and fast access of consultant pathologist to examined materiál even from oversea distance.
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