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Urogenital infections
JŮZOVÁ, Zdeňka
Bachelor thesis deals with infections affecting the urogenital tract of women in fertile age. The theoretical part is focused on all producers of urogenital infections causing complications for women, which include the total discomfort as in sex, difficulty in urination or postinflammatory complications for example infertility, but also discusses the adverse effects of infections on pregnancies and newborns. The practical part was conducted with professional assistance of staff in the microbiology laboratory synlab czech s.r.o in České Budějovice. There I learned to process sampled biological material (vaginal / cervical swab, urine), types of used agars to cultivation (UriSelect, KA, Sabouraud agar, GAR and MHA), various identification methods to confirm the microbe predetermined Dr. microbiologist according to macroscopic features and preparation with Gram-staining, among them Clumping factor, latex agglutination, PYR - test CAMP - test, IBK and instrument identification by MALDI - TOF. Then I made the cultivation for the presence of Mycoplasma hominis, Ureaplasma urealyticum and testing for the presence of antigen Chlamydia trachomatis by ELISA. And finally I performed the antimicrobial susceptibility testing with disk diffusion method. From processing of the results I found that among 148 women over a period of 1 to 18 September 2015 were most often found in samples of bacterial agens, particularly coagulase - negative Staphylococci, E. coli and Streptococcus agalactiae. I didn't find of Chlamydia trachomatis in any sample the selected group of women, Mycoplasma hominis was isolated only from one sample and Ureaplasma urealyticum was among five women.
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