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The development of methods in foreign language teaching from the mid 19th century until present
Hrušovská, Tereza ; Švermová, Dagmar (advisor) ; Nečasová, Pavla (referee)
This diploma thesis scrutinizes development of foreign language teaching methods as well as their conceptions and usage. The aim of this thesis is to provide a complex insight into the issue. The thesis works with a few hypotheses such as influence of age (of pupils as well as teachers) on choice of methods. Even more attention is dedicated to eclecticism and communicative methods which are believed to be predominant in today's teaching. The theoretical part is focused on explanation and clarification of methodologic terminology, all the methods and didactic approaches existing in foreign language teaching, which are presented chronologically. There are also influential psychological and linguistic movements important for development of the methods summarized. Then the research based on survey for teachers follows. This part explores which techniques, typical for particular methods, prevail in contemporary teaching. The aim of the research is to find out which methods and techniques are chosen by teachers and what factors influence their choice. The practical part functions as a sort of evidentiary test. There were designed two lessons for younger and older pupils which combine the survey results and authors personal teaching experience. The practical part shows if the preferences of teachers...
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Utilization of statistical approach in development of HPLC methods
Vymyslický, Filip ; Křížek, Tomáš (advisor) ; Kozlík, Petr (referee)
The aim of this diploma thesis was to develop a systematic procedure for the development of HPLC methods using the design of experiments. The system was developed based on the development of three HPLC methods for the determination of the purity of active substances using the design of experiments approach. The HPLC method for determining the purity of esomeprazole was selected to develop the systematic process for the development of HPLC methods by statistical approach. Experimental space was explored to find more suitable separation conditions. The second method used to develop the systematic procedure was the method for determining the purity of bisoprolol. This method was converted to a column of smaller size and the composition of the aqueous part of mobile phase was modified compared with the original pharmacopoeial method. Experimental space was explored to find more suitable separation conditions using the design of experiments. Last the method for determining the purity of risperidone was chosen. The composition of the aqueous part of mobile phase was changed in contrast to the original pharmacopoeial method. Experimental space was explored to find more suitable separation conditions using the design of experiments. For all studied HPLC methods, the values of the monitored chromatographic...
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Development of instrumentation and high-throughput screening methods for peptide ligand discovery and validation
Kryštůfek, Robin ; Konvalinka, Jan (advisor) ; Jiráček, Jiří (referee)
Peptides are used as synthetically available and easily derivatizable scaffold upon which it is possible to develop ligands targeting broad spectrum of biological targets. A time-tested approach to peptide binder identification is the preparation and screening of combinatorial libraries. Bypassing of this complicated procedure is possible by using biological systems for presentation, identification and selection of peptides based on the principle of in vitro evolution - i.e. display techniques. There are two complementary automated solutions for peptide binder identification described in this work. First is the SPENSER parallel peptide synthesizer, developed as a part of this diploma project, which can be used for peptide ligand discovery and optimization as well as validation of ligands identified using display techniques. Several libraries consisting of a total of 1 052 peptides have been prepared and then used to describe its potential applications. A sample of 154 preparations, representing 14.6 % analytical coverage of the prepared libraries, showed an average purity of 67 ± 19 % according to LC-MS. The libraries presented illustrate that SPENSER is a suitable tool for the parallel synthesis of linear and disulfide-cyclized peptides with limited variability, or libraries consisting of short...
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Utilization of statistical approach in development of HPLC methods
Vymyslický, Filip ; Křížek, Tomáš (advisor) ; Kozlík, Petr (referee)
The aim of this diploma thesis was to develop a systematic procedure for the development of HPLC methods using the design of experiments. The system was developed based on the development of three HPLC methods for the determination of the purity of active substances using the design of experiments approach. The HPLC method for determining the purity of esomeprazole was selected to develop the systematic process for the development of HPLC methods by statistical approach. Experimental space was explored to find more suitable separation conditions. The second method used to develop the systematic procedure was the method for determining the purity of bisoprolol. This method was converted to a column of smaller size and the composition of the aqueous part of mobile phase was modified compared with the original pharmacopoeial method. Experimental space was explored to find more suitable separation conditions using the design of experiments. Last the method for determining the purity of risperidone was chosen. The composition of the aqueous part of mobile phase was changed in contrast to the original pharmacopoeial method. Experimental space was explored to find more suitable separation conditions using the design of experiments. For all studied HPLC methods, the values of the monitored chromatographic...
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