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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Plasma-Assisted Deposition of Functional Bioactive Nanostructured Films
 SEZEMSKÝ, Petr
The Ph.D. thesis focuses on the interdisciplinary research of advanced materials for the development of biosensors with multiple domain interrogation capabilities for the detection of biomolecules and pathogens, namely, Lyme borreliosis spirochetes, Borrelia burgdorferi s.l. The strategy for biosensor fabrication is based on an optical fiber whose surface is coated by precisely tailored nanostructured thin films by means of low-temperature plasma. The thesis demonstrates that an indium tin oxide (ITO) thin film, deposited by magnetron sputtering onto the optical fiber surface, can serve as an effective transducer for electrochemical and optical measurements. The optical interrogation is based on the measurement of the refractive index utilizing lossy-mode resonance (LMR) phenomena, while the electrochemical interrogation is performed mostly by cyclic voltammetry. It is shown that the optimal design of the active surface on an optical fiber enables simultaneous (in time and in situ) optical and electrochemical interrogation of liquids and detection of biological complexes, including markers of Lyme borreliosis pathogens.
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