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Basic urine laborator examination
HAMOUZOVÁ, Kateřina
In this bachelor thesis I deal with the basic urine examination in the laboratory of clinical biochemistry. The bachelor thesis is mainly focused on the examination of urine chemically and the examination of urinary sediment. An essential part of the bachelor thesis is to approach public with the course of biological material examination in the laboratory. This work could be benefit for students or general public as an enghlitement of basic laboratory analysis of urine. Mainly in order to collect data which show the importance of testing, whether chemical or microscopic. The theoretical part briefly describes kidneys and their vascular supply. The division into primary and secondary urine is part of the chapter "Urine formation". Urine collection can be found in the same chapter. In the next chapter I present the chemical composition of urine. Urine composition plays an important role without any doubt in determining the patient's health. Furthermore, I characterize physical urine examination, which is an integral part of the basic urine examination. The next chapter is called Chemical examination of urine". Today, urine chemistry is performed through diagnostic strips. Last but not least, urine is examined microscopically. This examination is discussed in the "Penultimate chapter". In the last chapter I describe basic information about urinary stones. The methodology describes the procedure and principle of how to properly perform urine testing in the laboratory, whether chemically or microscopically. At the end of thecase report, the obtained data of analytes in the urine, which outlines the importance of the examined biological material.
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Testing of biological materials in surgeries of pediatricians - POCT
FRDLÍKOVÁ, Adéla
This thesis is concerned with the accumulation of results of the POCT diagnostic methods in a pediatrician's office and the results of measurements in a respective laboratory. I examined and gathered the results of three mostly tested biological materials which are blood, urine and stool. For the urinalysis, I conducted a basic chemical, microscopic and microbiological examination. When doing blood analysis, I determined the amount of C-reactive protein in blood. For stool analysis, I checked for the presence of rotaviruses and adenoviruses. The general description of the individual examinations is to be found in the theoretical part of this thesis. In methodology, I described the apparatus and methods in detail. To chemically analyze the urine, I worked with HarnUReader device which operates on the principle of photometrics. In a laboratory, I checked the provided results against HeptaPhan diagnostic strips and a microscope analysis. The presence of bacteria in the urine was established in the pediatrician's office by using the Uricult Trio cultivation tests, which are coated with a layer of agar on both sides. I confirmed these results in a laboratory by a bacterial inoculation on blood agar and Endo soil. In the pediatrician's office, I used the Quikread device which measures the amount of C-reactive protein in the specimen on the principle of photometrics. In the laboratory, I checked these results against the results made by the fully automated Architest plus c4000 device which works on the principle of turbidimetry. To detect the presence of rotaviruses and adenoviruses in the stool, I used the Diarlex MB set in the pediatrician's office and the Rapid-VIDITEST Rota Adeno set in the laboratory. This thesis accurately describes the course of action I took to work with the respective methods and explains the detected results. The results of my work in the pediatrician's office and in the laboratory are summarized in the discussion.
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