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Aspekty spermatologického vyšetření nativního bovinního ejakulátu
Weberová, Gabriela
In this bachelor thesis was described the reproductive system of a bull, hormonal regulation, sperm production and sperm, was also focused on the collection and sampling methods ejaculate. Work dealt with of studies of morphologically abnormal sperm and their influence on the fertility of bulls. At morphological defects been described primary and secondarily changed spermatozoa. Examination of native ejaculate was divided into microscopical, microbiological and special. At the end of this bachelor thesis was given the chapter on automated scoring systems ejaculates. Of those systems been described system for evaluating ejaculate CASA and flow cytometry.
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Hodnocení kvalitativních parametrů ejakulátů beranů
Valentová, Soňa
The aim of the thesis "Evaluation of qualitative parameters of rams ejaculates" was to compare techniques of ejaculate collection of rams of combined breed. The sampling technique for the artificial vagina was compared to that of the electro-ejaculator. The quantitative and qualitative indicators of ejaculates of rams were evaluated in the thesis. The parameters evaluated were: ejaculate volume, sperm movement, sperm initial aktivity, sperm concentration in ejaculate and sperm morphology. Samples were taken at four days. A total of 24 samples were obtained from 10 rams of the combined breed, of which 12 were sampled by artificial vagina sampling by stimulation with fixed sheep and 12 samples by electro-ejaculator. The electro-ejaculator collection technique was found to have lower sperm concentration (P≤0.01) and lower initial activity (P≤0.05) compared to the artificial vagina collection technique. The difference in ejaculate volume and sperm movement in the compared methods was not statistically demonstrated. No significant differences between sperm collection techniques in sperm abnormalities were observed or statistically demonstrated. The results of the diploma thesis showed that the better technique of ejaculate collection is taking on the artificial vagina, because it leads to higher initial activity of sperm (P≤0.05) and higher concentration of sperm (P≤0.01), which can be used mainly in the preparation of insemination doses.
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Aspekty spermatologického vyšetření nativního bovinního ejakulátu
Weberová, Gabriela
In this bachelor thesis was described the reproductive system of a bull, hormonal regulation, sperm production and sperm, was also focused on the collection and sampling methods ejaculate. Work dealt with of studies of morphologically abnormal sperm and their influence on the fertility of bulls. At morphological defects been described primary and secondarily changed spermatozoa. Examination of native ejaculate was divided into microscopical, microbiological and special. At the end of this bachelor thesis was given the chapter on automated scoring systems ejaculates. Of those systems been described system for evaluating ejaculate CASA and flow cytometry.
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