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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Interakce teploty a hormonů v růstu Arabidopsis thaliana
 Andrýsková, Kristýna
Abstract Due to global warming, plants are exposed to higher temperatures more often and for longer time periods. Exceeding the temperature optimum causes heat stress and affects plant growth and morphology. The most common plant reactions to high temperature are elongation of the hypocotyl, leaf hyponasty, or elongation of leaf petioles. All these morphological changes are collectively referred to as thermo-morphogenesis. The key modulator of this process is the transcription factor phy-tochrome-interacting factor PIF4. It is the main regulator of thermomorphogenesis through which the YUCCA8 gene is activated. YUCCA8 is a biosynthetic enzyme in auxin metabolism that induces auxin accumulation and activates cell elongation. The experimental part was focused on the influence of cytokinin hormones on the growth of the hypocotyl in Arabidopsis thaliana at standard (20 °C) and elevated (29 °C) temperatures. The increased temperature had a positive effect on the growth of the hypocotyl of control plants as well as transgenic lines with ectopic expression of the PIF4 gene. The effect of the application of 10µM trans-zeatin was temperature dependent. At standard temperature, cytokinins had a positive effect on the growth of the hypocotyl of control plants but did not have a significant effect in transgenic lines. However, transgenic lines had elongated hypocotyls compared to wild-type plants at standard temperature. Conversely, at a higher temperature, cytokinins only had an inhibitory effect. Automatic analysis of the abundance of the PIF4 pro-tein using GFP showed that its level within the hypocotyl is uneven with increasing abundance in the direction from the root to the cotyledons. Confocal microscopy confirmed that the inhibitory effect of cytokinins on hypocotyl elongation at higher temperatures is not due to a decrease in the PIF4 protein level.
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