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Rezistence dřepčíků a stonkových krytonosců na řepce k vybraným insekticidům
Richterová, Terezie
The aim of this thesis is to evaluate the selection of resistance to selected insecticides in populations of flea beetles and stem weevils collected from conventional oilseed rape fields in the Czech Republic during 2021 and 2022. Testing for resistance to the active ingredients lambda-cyhalothrin, tau-fluvalinate and acetamiprid was carried out according to the methods IRAC (Insecticide Resistance Action Committee) No. 011 and 021. The statistical program Polo Plus 2.0 was used to evaluate the tests, the percentage efficiencies, degrees of resistance and lethal doses were determined. Based on the results, it was found that the populations of flea beetles show resistance to lambda-cyhalothrin. Populations of the cabbage stem weevil show resistance to lambda-cyhalothrin and tau-fluvalinate. To the substance acetamiprid, the populations of stem weevils are currently sensitive. It is therefore necessary to apply anti-resistance strategies to these pests in order to prevent further selection of resistance.
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Vliv aplikace insekticidů na slunéčko východní (Harmonia axyridis)
Kintlerová, Jarmila
The diploma thesis was focused on evaluating the influence of selected active ingredients of insecticides used to protect agricultural crops on populations of the invasive species of harlequin ladybird (Harmonia axyridis Pallas, 1773) obtained from various locations within the Czech Republic. Four active ingredients, acetamiprid, indoxacarb, lambda-cyhalothrin and tau-fluvalinate, were tested according to the IRAC methodology on populations of adult individuals collected at seven locations. The experiment was done in the form of adult vial test, with subsequent evaluation using the Polo Plus 2.0 software. Based on the obtained results of mortality and lethal doses of active ingredients, it was found that the populations were highly sensitive to acetamiprid and lambda-cyhalothrin and sensitive with possible emergence of resistance to tau-fluvalinate. In the case of indoxacarb, populations from three locations showed moderate to high resistance.
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Rezistence blýskáčka řepkového (Brassicogethes aeneus, Fabr. 1775) k vybraným účinným látkám insekticidů
Langová, Melanie
The aim of this thesis was to evaluate the status of resistance of the pollen beetle (Brassicogethes aeneus, Fabr. 1775) to selected active ingredients of insecticides in 2020. The tested active ingredients were lambda-cyhalothrin, tau-fluvalinate, indoxacarb and Biscaya 240 OD (thiacloprid) according to IRAC testing methodology (Insecticide Resistance Action Committee) 011, 021 and 027 (adult vial test). After application of different concentrations of the insecticides were statistically evaluated their percent-age efficacy, degree of resistance of the population and lethal dose by Polo Plus 2.0 software. Based on the results, it was found that Brassicogethes aeneus populations showed resistance to lambda-cyhalothrin and tau-fluvalinate. Individuals were highly sensitive to indoxacarb. Brassicogethes aeneus showed considerable variability in the degrees of resistance against Biscaya 240 OD. Indoxacarb appears momentally to be the best way of plant protection, but further monitoring is important.
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