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Determination of the penetration of the active substances
 Blažejová, Natálie ; Kalina, Michal (referee) ; Klučáková, Martina (advisor)
This thesis is focused on the simulation of penetration of active ingredients used in cosmetic products. A theoretical part was written which focused on the basic characteristics of active ingredients used in the cosmetic and pharmaceutical industry. A method for the determination of these actives by means of Franz diffusion cells, which are used to determine the concentration of actives passed through the skin using different skin models, was described. The experimental part focused on the simulation of the penetration of two active ingredients, bisabolol and ectoine, which were provided by the cosmetic company Nobilis Tilia for this work. The amount of these actives was determined through Franz diffusion cells, using a synthetic Strat-M® membrane and a membrane prepared from pig ear skin. Furthermore, the tape-stripping method was used to determine the active substance in the skin layers. All samples were then analysed by UV-VIS spectrometry and liquid chromatography.
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