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Dissemination of Cordyceps fumosorosea by entomopathogenic Nematodes
HOPFGARTNER, Miriam
C. fumosorosea is an entomopathogenic fungus that has been used as a biocontrol agent against various insect hosts for some time. Its efficacy as an entomopathogen can be increased by joined application of the fungus with entomopathogenic nematodes. The aims of this thesis are to investigate the impact of the foraging strategy of different nematode species on the spore dispersal of C. fumosorosea and the significance the nematode's 2nd stage cuticle holds for spore dissemination. Additionally, novel approaches for the enhancement of spore transmission in soil environments via adhesives are explored.
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Nucleotide variability in \kur{enol-1} gene of entomopathogenic nematodes \kur{Heterorhabditis bacteriophora} and \kur{Steinernema feltiae}
HEJNÍČKOVÁ, Martina
The topic of this bachelor thesis is enol-1, parasitism-related gene in entomopathogenic nematodes. First part of this thesis deals with pattern of the enol-1 nucleotide variability. Nucleotide sequence of enol-1 was obtained from 9 strains of Heterorhabditis bacteriophora and 8 strains of Steinernema feltiae. I characterised and compared its structure and nucleotide variability. Phylogenetic analysis of enol-1 was done with closely related nematode coding sequences, acquired from internet databases. The second part of this thesis is devoted to virulence testing. Larvae of Galleria mellonella were infected with different strains of entomopathogenic nematodes, and their mortality was summarized.
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Standard bioassay of the efficacy of entomoparazitic nematodes
TRNKOVÁ, Zuzana
The efficacy of three entomopathogenic nematodes (EPN) of family Steinernematidae was tested in different laboratory conditions in this study. The virulence of Steinernema arenarium, S. carpocapsae and S. feltiae was evaluated to fourth-instar larvae of Galleria mellonella and Tenebrio molitor. Standard temperature (25°C) and humidity (12,5 %) was used in bioassay No I. Trial No II focused on impact of temperature 10, 15 and 25°C on effectivity of S. feltiae and S. carpocapsae. Bioassay No III examined the influence of lower humidity and in consequence rehydration on virulence nematodes.
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Synergism of entomopathogenic nematodes and entomopathogenic fungi
ŠILLEROVÁ, Tereza
The potential synergism between chosen species of entomopathogenic nematodes (Steinernema arenarium, Steinernema feltiae) and entomopathogenic fungi (Beauveria bassiana, Isaria fumosorosea, Lecanicillium lecanii) is investigated in this study. It is theoretically possible to expect increasing of their efficiency at the collective introduction into environment. Creating of uniform laboratory method which will be possible to use at the research of this interaction system is a part of this study.
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