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The issue of urgent diagnostics of tick-borne encephalitis
MYSLIVCOVÁ, Lenka
In my bachelor thesis I dealt with urgent diagnostics of tick-borne encephalitis in the hospital of Havlíčkův Brod. The practical part was carried out in the immunology laboratory in the Joint Laboratories Unit in the hospital of Havlíčkův Brod, where the ChorusTrio device was purchased at the end of 2018. This device accelerated the diagnosis of samples from patients with suspected tick-borne encephalitis. Unfortunately, this device is only suitable for screening sera and is therefore mainly used to control antibodies before or after vaccination. In 2019 a new device, the ReaScan rapid test, was purchased and used for statim diagnostics. It is a simple and fast immunochromatographic test that allows us to confirm or refute the diagnosis of tick-borne encephalitis within 30 minutes. In the theoretical part I elaborated professional literature about tick-borne encephalitis, tick-borne encephalitis virus, its diagnostics and other related topics. In the practical part I dealt with methods whose principles are described in the theoretical part, in the chapter Laboratory diagnostics of tick-borne encephalitis in the hospital of Havlíčkův Brod, and whose working procedures are described in the chapter Methodology. From the obtained data and from the data from the LIS (Laboratory Information System) I compiled statistics, in which I evaluated the rate of return of the results from the laboratory examination. The aim of my bachelor thesis was to evaluate the existing statim diagnostics of tick-borne encephalitis with special emphasis on TAT (time of return of the examination results) and to find possible ways how to improve the diagnostics.
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