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National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Laboratotry diagnosis of intestinal protozoa with a focus on Dientamoeba fragilis
 Vyleťalová, Lea ; Vejsová, Marcela (advisor) ; Konečná, Klára (referee)
CHARLES UNIVERSITY Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Medical Laboratory Technician Bachelor's thesis Author: Lea Vyleťalová Supervisor: Mgr. Marcela Vejsová, Ph.D. Title: Laboratory diagnosis of intestinal protozoa with a focus on Dientamoeba fragilis Infections caused by intestinal protozoa are currently a persistent health problem. These infections mainly affect populations in developing countries due to their limited laboratory facilities, often poor hygiene practices and limited funding for health services. Although traditional diagnostic methods based on microscopy and staining are still in use, they present significant limitations in terms of low sensitivity and specifity, as well as time- consuming and the need for trained laboratory workers. For this reason, new methods are still being developed in developed areas, particularly in the field od molecular biology. These methods are based on the principle of the polymerase chain reaction and its multiplex design. Other possible methods suitable for the detection of intestinal parasitic infections include immunological and culture-based methods. One of the intestinal parasitic protozoa causing gastrointestinal disease is the cosmopolitan trichomonad Dientamoeba fragilis. This is a parasite whose...
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