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Voltammetric Determination of Nimesulide at a Polished Silver Solid Amalgam Electrode
Koščová, Kristína ; Vyskočil, Vlastimil (advisor) ; Fischer, Jan (referee)
This bachelor thesis focuses on investigating the electrochemical behavior of the substance nimesulide. The stability of the stock solution was monitored using UV-VIS spectrophotometry. A solid silver amalgam electrode with a polished surface was chosen as the working electrode, a silver chloride reference electrode (3M KCl), and a platinum auxiliary electrode were used. For the techniques of DC voltammetry (DCV) and differential pulse voltammetry (DPV), optimal conditions for the determination of nimesulide were sought (optimal pH, range of regenerative potentials). The calibration dependence was measured in concentration ranges of 10−7 , 10−6 , and 10−5 mol/L. In the case of DCV, a linear calibration dependence was achieved in the concentration range of 1-100 μmol/L (with a limit of detection (LD) ≈ 0.27 μmol/L and with a limit of quantification (LQ) ≈ 0.91 μmol/L). In the case of DPV, linearity was achieved in the range of 4-100 μmol/L (LD ≈ 0.60 μmol/L and LQ ≈ 2.0 μmol/L). The developed voltammetric methods were used to determine nimesulide in drinking and well water and in pharmaceutical forms of Nimesil 100 mg and Aulin 100 mg.
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