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Influence of leaf age on the Rubisco content
Matulková, Zuzana ; Zemanová, Jana (referee) ; Hrstka, Miroslav (advisor)
The bachelor’s thesis describes the influence of leaf senescence on the Rubisco enzyme content in Fagus sylvatica, grown both in normal and elevated athmospheric carbon dioxide concentration. Tha samples were collected at the Station of experimental ecology, Institute of systems biology and ecology, Academy of Sciences of the Czech Republic, White Cross in Moravian-Silesian Beskydy. To specify the determination three series of samples were taken in following order - the first one on 22nd May 2007, the second one on 24th July 2007, and the third one then on 4th September 2007.The Rubisco enzyme content in leafs was measured by the SDS-PAGE method. In Fagus species grown in elevated carbon dioxide concentration we observed the depression of Rubisco enzyme content during the vegetation period. Reversely in Fagus species grown in normal carbon dioxide concentration the Rubisco enzyme content was increasing.

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