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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Measurement of Spectral Characteristics of Fluorescent Voltage-Sensitive Dyes
 Pavlík, Dušan ; Čmiel, Vratislav (referee) ; Provazník, Ivo (advisor)
This bachelor thesis deals with optical measurement of substances properties in practical service of absorption and fluorescent spectroscopy. The work specifies the principle of fluorescence, which has direct association with spectral characteristics measurement of so-called voltage-sensitive dyes (in this instance di-4-ANEPPS). This dye is used for contactless (optical) imaging of organisms electric activity in practical service of its voltage dependent spectral shift after binding itself on cell membrane. However, the right application of this fluorescent dye is dependent on too many factors – one of them, discussed and measured in this work, is di-4-ANEPPS spectral dependency on its immediate environment.
Detailed record
Measurement of Spectral Characteristics of Fluorescent Voltage-Sensitive Dyes
 Pavlík, Dušan ; Čmiel, Vratislav (referee) ; Provazník, Ivo (advisor)
This bachelor thesis deals with optical measurement of substances properties in practical service of absorption and fluorescent spectroscopy. The work specifies the principle of fluorescence, which has direct association with spectral characteristics measurement of so-called voltage-sensitive dyes (in this instance di-4-ANEPPS). This dye is used for contactless (optical) imaging of organisms electric activity in practical service of its voltage dependent spectral shift after binding itself on cell membrane. However, the right application of this fluorescent dye is dependent on too many factors – one of them, discussed and measured in this work, is di-4-ANEPPS spectral dependency on its immediate environment.
Detailed record

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