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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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Quantification of specific microbial functional guilds in Arctic permafrost soil
WALTER, Deborah
The quantity of specific bacterial and archaeal groups were determined in permafrost soils. The selected groups were quantified by quantitative polymerase chain reaction (qPCR) of specific genes: total archaea (16SrRNA gene), methanogenic archaea (mcrA gene), nitrogen fixators (nifH gene) and denitrifiers (nosZ gene). The quantities of these groups were compared between different soil horizons and furthermore correlations between the quantity of gene copies and basic soil parameters were computed.
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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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