|
|
Analysis of metabolites produced by yeast colonies and biofilms
Karásek, Filip ; Palková, Zdena (advisor) ; Dostál, Jiří (referee)
Some time ago it was shown (proved, described) that bacteria can communicate and regulate a gene expression by using small molecules. This phenomenon is called quorum sensing. The same phenomenon has been recently described in yeast. In yeast there are still many questions about the mechanism of sensing via small molecules. Few works and publications have described the impact of some small molecules, such as phenylethylalcohol or tryptophol, on morphology on Saccharomyces cerevisiae cell. The knowledge about the impact of the small molecules on the yeast multicellular community, like colonies, is still very limited these days. The aims of this work are analysis of exometabolites produced by colonies of different yeast strains cultivated on various solid complex media, by an untarged metabolomic approach. In accordance with the result of the exometabolites analyses we are going to test the impact of the selected substance on Saccharomyces cerevisiae colony morphology. The third part of this work is about the construction of Saccharomyces cerevisiae strains with deletion or lowering the expression of genes which encode proteins that are important in decarboxylation step of the Ehrlich pathway. Key word: Saccharomyces cerevisiae, microcolonies, tryptophol, isoamyl alcohol, isovaleric acid, colony...
|
guest ::
