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Preparation of recombinant PsbQ protein of photosystem II from spinach for studies on the interactions between oxygen-evolving complex proteins.
HELLER, Jiří
Proteins of the oxygen-evolving complex of photosystem II from spinach are studied to gain information about the interactions between the participating proteins and thus structural insight into its role in the mechanism underlying oxygenic photosyntesis in higher plants. The work describes in detail the recombinant expression and purification of PsbQ in Escherichia coli, and site-directed mutagenesis of the W71F and W71Y mutants as candidates for protein interactions studies.

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