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Vliv umělé inokulace na druhové složení ektomykorhizních hub semenáčků borovice lesní
 Loubalová, Karolína
This bachelor thesis deals with the influence of artificial inoculation on biometric parameters and on the occurrence of ectomycorrhiza on the root system of Scots pine seedlings in the forest nursery Svinošice. Differences between measured biometric parameters of seedlings for each treatment were tested by analysis of variance (ANOVA). Ectomycorrhizas were sorted on the basis of the morphological characteristics into so-called morphotypes; ectomycorrhizal fungi forming individual morphotypes were identified by comparing of sequences of the ITS rDNA. These observed data were processed with statistical program Canoco. The inoculation was unsuccessful, fungi used for this inoculation did not form mycorrhiza on seedlings. Representation of the ectomycorrhiza morphotypes on the roots of the seedlings had no statistically significant effect on their biometric parameters. Positive results of this work consist in clearly described and validated methodology for identification of ectomycorrhizal fungal symbionts via the description of mycorrhiza morphotypes and subsequent method of comparison of ITS rDNA sequences.
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