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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Molecular analysis of transgenic rhododendron plants obtained by transformation with 35SGUSint. construct
 SKOTNICOVÁ, Petra
The goal of this thesis was molecular analysis of rhododendron plants obtained after transformation by Agrobacterium tumefaciens and subsequent selection on kanamycin. Presence of incorporated genes gusA and nptII was determined by polymerase chain reaction (PCR) and Southern analysis. GUS activity was determined by fluorimetric and histochemical assay too.
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The molecular analysis of transgenic \kur{Lycopersicon esculentum} plants harbouring incorporated pmi gene for phosphomannose isomerase
 PŘIKRYLOVÁ, Pavla
The aim of this study was to analyze number of incorporated T-DNA harbouring pmi and nptII transgenes and expression of the pmi gene in tomato transformants using Southern blot and Northern blot methods. The presence of a functional PMI protein was assesed using PMI-assay with Chlorophenol red dye. Mannose selection of tomato seeds was established and segregation patterns in T1 progeny were studied.
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