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Characterization of lipids in cheese analogues
Bittnerová, Eva ; Šimíčková, Adéla (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the identification and quantification of free and bound fatty acids in samples of vegetable oils, specifically in linseed and rapeseed, and in cheese analogues made from them. The theoretical part deals with the characterization of cheese analogues, the composition and technology of fresh cheese production and the description of selected vegetable oils. It also contains the principle and instrumentation of gas chromatography with a flame ionization detector. In the experimental part, model samples of fresh cheese analogues with the addition of linseed and rapeseed oil and the corresponding oil as the basic raw material for their production were analyzed. The fat was extracted from the cheese analogues with a mixture of diethylether and petroleumether, the fatty acids were converted to methylesters by acid esterification with methanolic bortrifluoride catalyst and determined by gas chromatography with flame ionization detection. A total of 15 fatty acids were identified, predominantly oleic, linolenic and palmitic acids. In both oils, oleic, linoleic and linolenic acids were the most represented; significant amounts of myristic, palmitic and stearic acids were also found in the analog samples. The content of free fatty acids was significantly lower than that bound of all samples.
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Characterization of lipids in cheese analogues
Bittnerová, Eva ; Šimíčková, Adéla (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the identification and quantification of free and bound fatty acids in samples of vegetable oils, specifically in linseed and rapeseed, and in cheese analogues made from them. The theoretical part deals with the characterization of cheese analogues, the composition and technology of fresh cheese production and the description of selected vegetable oils. It also contains the principle and instrumentation of gas chromatography with a flame ionization detector. In the experimental part, model samples of fresh cheese analogues with the addition of linseed and rapeseed oil and the corresponding oil as the basic raw material for their production were analyzed. The fat was extracted from the cheese analogues with a mixture of diethylether and petroleumether, the fatty acids were converted to methylesters by acid esterification with methanolic bortrifluoride catalyst and determined by gas chromatography with flame ionization detection. A total of 15 fatty acids were identified, predominantly oleic, linolenic and palmitic acids. In both oils, oleic, linoleic and linolenic acids were the most represented; significant amounts of myristic, palmitic and stearic acids were also found in the analog samples. The content of free fatty acids was significantly lower than that bound of all samples.
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