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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Prostorové šíření vzdušného inokula patogenní houby Fusarium circinatum na infikované lokalitě v Galícii
 Janoš, Patrik
The aerial inoculum of the Fusarium Circinatum, a fungus from the family of Ascomycota, which causes the Pine Pitch Cancer (PPC), is one of the primary disseminators of the destructive tree disease in forest stands and nurseries. Despite the fact that this pathogen is widely known nowadays, its biological characteristics are to some extent still largely unexplored, even after its introduction to Europe and the subsequent emerging of newly infected areas. In an attempt to shed more light on this topic, we conducted an experiment in the Pinus monterey and Pinus pinaster forest stands in Galicia (Spain) that were infested with the pathogen. The objective was to provide a detailed description of the spatial dissemination of the F. circinatum spores depending on the orientation and the velocity of wind. To detect and quantify the aerial inoculum of the pathogen, rotational spore traps combined with quantitative real-time PCR were employed. In three out of four conducted observations, the impact of the wind orientation with the velocity of 5 m/s manifested as a positive factor for the dissemination. The inoculum presence was detected within the distance of 1000 meters from the infected area following the draft of the wind. Our research has therefore confirmed that the wind orientation and velocity positively influence the dissemination of the pathogen spores. At the same time the study demonstrated that the combination of rotational spore traps and qPCR can be an eligible tool for the detection of F. circinatum. The results of the experiment were affected by low concentration of inoculum during the time period of the experiment, as well as by lowered reliability of the data concerning the orientation and velocity of wind.
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