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Diversity of Polycomb complexes and their function
ŘÍHA, Luboš
The aim of this bachelor thesis is testing and further development of a vector system that should help clarify the alleged functional redundancy of Polycomb repressive complex 2 (PRC2) subunits. The theoretical part introduces the field of epigenetics and the role of Polycomb group complexes (PcGs) in Arabidopsis thaliana (mouse-ear cress) is explained. The issue of PRC2 subunits SWN and CLF redundancy is set in context and the tested hypothesis is explained. Genetic engineering tools relevant to this study are presented. Finally, the background of the promoter and marker vectors developed in the practical part is explained. In the practical part vectors with markers and promoters are developed and transgenic plants were grown on selection and genotyped. Results are presented and discussed.

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