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Use of insemination in beef cattle herds
JANIŠ, Marek
This bachelor thesis deals with the evaluation of the use of insemination in a selected herd of beef cattle. The thesis is divided into two parts, theoretical and practical. The theoretical part of the bachelor thesis characterizes the concepts related to the topic.It describes the anatomy and physiology of the cow, insemination and methods, genomics and current and future trends in the field of insemination. The practical part interprets and evaluates the results of the analysis, which are based on the cow's passport cards and the record of the performance check.
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Effect of zearalenone on reproductive parameters and the expression of selected genes in mice.
Dvořáková, Eva ; Pěknicová, Jana (advisor) ; Nedvídek, Josef (referee)
A number of chemicals may have a negative impact on the environment and wildlife. Endocrine disruptors (EDs), which can mimic estrogen, interfere with natural hormones in organism and can have a negative effect on the reproductive system. Such substances include zearalenone (ZEA) - mycotoxin, produced by the fungi Fusarium. Despite the non-steriodal structure of its molecule, ZEA and its derivates possess potent estrogenic activity. The influence of ZEA on reproductive parameters and changes in expression of selected genes were tested in the outbred line of mice. This study showed significant effects of ZEA on number of reproductive performances. This effect was observed at lower examined dose, to which are humans normally exposed, and at higher doses, both showed changes in the tested parameters.
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The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in diabetic mice
Valášková, Eliška ; Pěknicová, Jana (advisor) ; Kaňka, Jiří (referee)
According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. Causes of this condition could be genetic background, environmental factors and various diseases, including diabetes mellitus (DM). The aim of this study was to investigate the effects of DM on reproductive parameters and expression of selected testicular genes using mouse model (FVB inbred mouse strain). DM (type 1) was artificially induced by chemical substance streptozotocin, which causes destruction of pancreatic β cells. These mice were exposed to diabetic condition for 6 weeks and then subjected to analysis. Our results have shown that diabetic condition had an impact on body weight, weight of reproductive organs as well as kidneys and livers. We also observed decreased concentration and viability of diabetic sperm compared to control. Moreover, we noticed increased staining with apoptotic marker annexin V. Further, we evaluated changes of sperm nuclear proteins - protamines. In diabetic animals, we observed higher number of sperm with insufficient protamination. Nevertheless, protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was not altered in sperm of diabetic animals compared to control. Regarding the...
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The effect of diabetes mellitus on reproductive parameters and expression of selected testicular genes in diabetic mice
Valášková, Eliška ; Pěknicová, Jana (advisor) ; Kaňka, Jiří (referee)
According to the World Health Organization (WHO), 15% of couples in reproductive age suffer from infertility problems, and up to 60% of cases are caused by male factor. Causes of this condition could be genetic background, environmental factors and various diseases, including diabetes mellitus (DM). The aim of this study was to investigate the effects of DM on reproductive parameters and expression of selected testicular genes using mouse model (FVB inbred mouse strain). DM (type 1) was artificially induced by chemical substance streptozotocin, which causes destruction of pancreatic β cells. These mice were exposed to diabetic condition for 6 weeks and then subjected to analysis. Our results have shown that diabetic condition had an impact on body weight, weight of reproductive organs as well as kidneys and livers. We also observed decreased concentration and viability of diabetic sperm compared to control. Moreover, we noticed increased staining with apoptotic marker annexin V. Further, we evaluated changes of sperm nuclear proteins - protamines. In diabetic animals, we observed higher number of sperm with insufficient protamination. Nevertheless, protamine 1 to protamine 2 ratio (P1/P2), a marker of male fertility, was not altered in sperm of diabetic animals compared to control. Regarding the...
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Effect of zearalenone on reproductive parameters and the expression of selected genes in mice.
Dvořáková, Eva ; Pěknicová, Jana (advisor) ; Nedvídek, Josef (referee)
A number of chemicals may have a negative impact on the environment and wildlife. Endocrine disruptors (EDs), which can mimic estrogen, interfere with natural hormones in organism and can have a negative effect on the reproductive system. Such substances include zearalenone (ZEA) - mycotoxin, produced by the fungi Fusarium. Despite the non-steriodal structure of its molecule, ZEA and its derivates possess potent estrogenic activity. The influence of ZEA on reproductive parameters and changes in expression of selected genes were tested in the outbred line of mice. This study showed significant effects of ZEA on number of reproductive performances. This effect was observed at lower examined dose, to which are humans normally exposed, and at higher doses, both showed changes in the tested parameters.
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Analysis of production traits in broiler hens and their offspring
FARA, Jiří
The aim of the bachelor thesis was to compare the production traits of parental combination and their progeny of commercial broiler chicken genotypes Cobb 500 and Ross 308. The data was obtained from the International Poultry Testing Station in Ústrašice. Parental test consisted of parent rearing until the age of 154 days, the laying period of 280 days and from four fattening progeny tests (lasting for 35 days or else 42 days). Ross 308 chickens reached the higher live weight compared to Cobb 500 at the end of the rearing period in parental test. The lower feed consumption per head and day had Cobb 500 hybrids during this period. The lower feed consumption per day and per 1 chicken was found in Cobb 500 hybrids in the laying period. The higher eggs number/hen housed was laid by Ross 308 hens. Number of hatched chickens per laying hen was higher in Cobb 500 hens. Higher live weight and lower feed consumption per 1 kilogram gain hybrids was found in Ross 308 at fattening test at 35 days of age. Cockerels of both hybrid combinations reached higher live weight and lower feed consumption per kilogram gain. 2.3 % chickens died during the fattening period in each hybrid combination. The higher carcass yield and breast and thighs muscle weight were determined in Ross 308 hybrids. Ross 308 hybrids achieved higher live weight, only about 14 g at longer fattening test at 42 days of age. Higher live weigh and lower feed consumption was fond out in cockerels in comparison with pullets in both genotypes. 4.6 % Cobb chickens and 5.4 % Ross 308 chickens died during the fattening period (ventilation problems). The difference between genotypes Ross 308 and Cobb 500 was only 0.2 % in carcass yield, 7 g in breast weight and 9 g in thighs weight.
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