National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
Proteome dynamics during seed germination
Habánová, Hana
Seed germination is a crucial phase of the plant life cycle. Despite the huge progress that has been made in the last decade, the molecular mechanisms regulating seed germination are far from being resolved. Most of the early events of germination are mediated by molecules stored in seeds during the seed development and thus we employed proteomic analyses to elucidate the mechanism behind this process. This thesis - Proteome dynamics during seed germination - reviews present-day knowledge of this topic. In the experimental part, the results of three related research projects are summarized. First, the effects of a novel class of growth regulators were analyzed in the barley germination assay and the optimal concentrations were determined. The methodology for an improved analysis of barley seed proteins was developed and the optimized targeted analysis will allows the detection of over 4,000 proteins. Finally, Arabidopsis thaliana seed proteome was analyzed and the results indicate that (i) an alternative MS/MS data processing significantly improves the detection limits and (ii) the proteasome-ubiquitin system plays a crucial role in adjusting the seed proteome dynamics during the germination progress.
Využití regulátorů růstu v ječmeni jarním k omezení poléhání
Tvrdý, Hynek
This thesis deals with use of growth regulators to reduce lodging of spring barley (Hordeum vulgare L.). The field trial was conducted in corn production area on a clay-loamy glue fluvisol since 2012 to 2014. An effect of growth regulators was monitored on spring barley of the variety Bojos. Preparations Retacel extra R 68 (chlormequat chloride), Moddus (trinexapac-ethyl) and Cerone 480 SL (etephon) were chosen to crop regulation. The aim of the thesis was to carry intensive crop barley focused on correct application of growth regulators according to the cultivation methodologies. Growth regulators were used in various combinations with one another in three dose and dosage times. From this reason cob length, number of seeds per spike, grain yield and selected parameters of malting barley quality were monitored and evaluated. There was no negative effect of different combinations and does of growth regulators on production and quality of spring barley while the aim of crop shortening was achieved. In 2014 up to 20% of the effect of stalk length shortage was achieved. The greatest shortening was achieved on the stalks with higher dose of Cerone. In 2013 the yield increased of 1,95 t.ha-1 in variants with growth regulators.

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