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National Repository of Grey Literature 1 records found  Search took 0.01 seconds. 
Optimization of methods for evaluation of antimicrobial activity of selected active matters against chosen spectrum of bacterial pathogens
 BRZÁKOVÁ, Michaela
The bachelor thesis deals with optimalization of selected methods (e.g. evaluation of bacterial suspension concentration, Resazurin antibacterial assay) needed for evaluation of active substances antibacterial activity against pathogens Pectobacterium carotovorum, Pseudomonas syringae and Clavibacter michiganensis subsp. michiganensis. Literature review contains information about potato tubers proteins, especially proteins with antibacterial activities, as well as a brief overview of mechanisms of action of plant defense system against pathogenic microorganisms and the effect of antimicrobial substances on bacterial pathogens. The experimental part deals with optimalization of methods needed for evaluation of antibacterial activities of selected substances. Optimized were methods of McFarland Turbidity Standards, Most-probable number method and Resazurin antibacterial assay. It was found that increase of optical density, measured by spectrometer at 620 nm, is in direct correlation with increase of bacterial suspension concentration (CFU/ml). It was identified proteins of patatin complex and protease inhibitors by using SDS-PAGE method to evaluated potato tuber proteins of variety Bella and Westamyl and also changes of molar mass of the patatin proteins in relation of their modification were evaluated. Antimicrobial activity of these proteins was verified by resazurin antibacterial assay and ability of modified proteins of cultivars Bella and Westamyl in higher concentration (10 mg/ml) to inhibit growth of bacteria Pseudomonas syringae.
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